Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles
Historically efforts have focused on the human leukocyte antigen (HLA) as the major cause for acute and chronic rejection following cardiac transplantation. However, rising evidence indicates that non-HLA antibodies can be both primary initiators and modifiers of antibody-mediated rejection (AMR) an...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2017-10-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01332/full |
| _version_ | 1819131784524201984 |
|---|---|
| author | Katherine V. Gates Katherine V. Gates Naveen L. Pereira Leigh G. Griffiths |
| author_facet | Katherine V. Gates Katherine V. Gates Naveen L. Pereira Leigh G. Griffiths |
| author_sort | Katherine V. Gates |
| collection | DOAJ |
| description | Historically efforts have focused on the human leukocyte antigen (HLA) as the major cause for acute and chronic rejection following cardiac transplantation. However, rising evidence indicates that non-HLA antibodies can be both primary initiators and modifiers of antibody-mediated rejection (AMR) and cardiac allograft vasculopathy (CAV). The purpose of this review is to assess currently available technologies for non-HLA identification and leveraging such responses toward antibody quantification. Several techniques have been used to identify antigenic determinants of recipient graft-specific non-HLA humoral immune responses, but each comes with its own set of benefits and caveats. Improving our ability to detect non-HLA humoral immune response will aid in our understanding of the underlying antigenic determinants of AMR and CAV, as well as improve patient outcomes. |
| first_indexed | 2024-12-22T09:21:01Z |
| format | Article |
| id | doaj.art-e9cdd9425a17428499471906fa7e9d3b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T09:21:01Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e9cdd9425a17428499471906fa7e9d3b2022-12-21T18:31:12ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-10-01810.3389/fimmu.2017.01332296813Cardiac Non-Human Leukocyte Antigen Identification: Techniques and TroublesKatherine V. Gates0Katherine V. Gates1Naveen L. Pereira2Leigh G. Griffiths3Department of Veterinary Medicine and Epidemiology, University of California, Davis, Davis, CA, United StatesDepartment of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, United StatesDepartment of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, United StatesDepartment of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, United StatesHistorically efforts have focused on the human leukocyte antigen (HLA) as the major cause for acute and chronic rejection following cardiac transplantation. However, rising evidence indicates that non-HLA antibodies can be both primary initiators and modifiers of antibody-mediated rejection (AMR) and cardiac allograft vasculopathy (CAV). The purpose of this review is to assess currently available technologies for non-HLA identification and leveraging such responses toward antibody quantification. Several techniques have been used to identify antigenic determinants of recipient graft-specific non-HLA humoral immune responses, but each comes with its own set of benefits and caveats. Improving our ability to detect non-HLA humoral immune response will aid in our understanding of the underlying antigenic determinants of AMR and CAV, as well as improve patient outcomes.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01332/fullproteomicsimmunoproteomicsnon-human leukocyte antigen antibodyantigen identificationheart transplantation |
| spellingShingle | Katherine V. Gates Katherine V. Gates Naveen L. Pereira Leigh G. Griffiths Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles Frontiers in Immunology proteomics immunoproteomics non-human leukocyte antigen antibody antigen identification heart transplantation |
| title | Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles |
| title_full | Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles |
| title_fullStr | Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles |
| title_full_unstemmed | Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles |
| title_short | Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles |
| title_sort | cardiac non human leukocyte antigen identification techniques and troubles |
| topic | proteomics immunoproteomics non-human leukocyte antigen antibody antigen identification heart transplantation |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01332/full |
| work_keys_str_mv | AT katherinevgates cardiacnonhumanleukocyteantigenidentificationtechniquesandtroubles AT katherinevgates cardiacnonhumanleukocyteantigenidentificationtechniquesandtroubles AT naveenlpereira cardiacnonhumanleukocyteantigenidentificationtechniquesandtroubles AT leighggriffiths cardiacnonhumanleukocyteantigenidentificationtechniquesandtroubles |