Alternative method of allelic discrimination
5′ nuclease assays for allelic discrimination use both a wild-type and a mutant probe. Here we present a new method for genotyping by 5′ nuclease assays that dispenses with the mutant probe, using the wild-type probe together with a probe for a reference gene known to be present in two copies to det...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2014-08-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114199 |