Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) Protein
The effects of chemical protein extraction, and enzymatic hydrolysis with Alcalase, papain and pepsin, on the functional properties, antioxidant activity, amino acid composition and protein structure of black soldier fly (<i>H. illucens</i>) larval protein were examined. Alcalase hydroly...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/12/876 |
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| author | Inayat Batish Devon Brits Pedro Valencia Caio Miyai Shamil Rafeeq Yixiang Xu Magdalini Galanopoulos Edward Sismour Reza Ovissipour |
| author_facet | Inayat Batish Devon Brits Pedro Valencia Caio Miyai Shamil Rafeeq Yixiang Xu Magdalini Galanopoulos Edward Sismour Reza Ovissipour |
| author_sort | Inayat Batish |
| collection | DOAJ |
| description | The effects of chemical protein extraction, and enzymatic hydrolysis with Alcalase, papain and pepsin, on the functional properties, antioxidant activity, amino acid composition and protein structure of black soldier fly (<i>H. illucens</i>) larval protein were examined. Alcalase hydrolysates had the highest degree of hydrolysis (<i>p <</i> 0.05), with the highest hydrolysate and oil fraction yield (<i>p</i> < 0.05). Pepsin hydrolysates showed the lowest oil holding capacity (<i>p</i> < 0.05), whereas no significant differences were observed among other enzymes and protein concentrates (<i>p</i> > 0.05). The emulsifying stability and foam capacity were significantly lower in protein hydrolysates than protein concentrate (<i>p</i> < 0.05). The antioxidant activity of protein hydrolysates from protein concentrate and Alcalase was higher than that with papain and pepsin (<i>p</i> < 0.05), owing to the higher hydrophobic amino acid content. Raman spectroscopy indicated structural changes in protein α-helices and β-sheets after enzymatic hydrolysis. |
| first_indexed | 2024-03-10T14:14:05Z |
| format | Article |
| id | doaj.art-e9db359b940e481d97b01f9801594fdf |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T14:14:05Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-e9db359b940e481d97b01f9801594fdf2023-11-21T00:00:22ZengMDPI AGInsects2075-44502020-12-01111287610.3390/insects11120876Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) ProteinInayat Batish0Devon Brits1Pedro Valencia2Caio Miyai3Shamil Rafeeq4Yixiang Xu5Magdalini Galanopoulos6Edward Sismour7Reza Ovissipour8Department of Food Science and Technology, Virginia Tech, Blacksburg, VA 24060, USADepartment of Entomology, Louisiana State University, Baton Rouge, LA 70803, USADepartment of Chemical and Environmental Engineering, Technical University Federico Santa María, Valparaíso 2340000, ChileVirginia Seafood Agricultural Research and Extension Center, Virginia Tech, Hampton, VA 23669, USAVirginia Seafood Agricultural Research and Extension Center, Virginia Tech, Hampton, VA 23669, USAAgricultural Research Station, Virginia State University, Petersburg, VA 23806, USAAgricultural Research Station, Virginia State University, Petersburg, VA 23806, USAAgricultural Research Station, Virginia State University, Petersburg, VA 23806, USADepartment of Food Science and Technology, Virginia Tech, Blacksburg, VA 24060, USAThe effects of chemical protein extraction, and enzymatic hydrolysis with Alcalase, papain and pepsin, on the functional properties, antioxidant activity, amino acid composition and protein structure of black soldier fly (<i>H. illucens</i>) larval protein were examined. Alcalase hydrolysates had the highest degree of hydrolysis (<i>p <</i> 0.05), with the highest hydrolysate and oil fraction yield (<i>p</i> < 0.05). Pepsin hydrolysates showed the lowest oil holding capacity (<i>p</i> < 0.05), whereas no significant differences were observed among other enzymes and protein concentrates (<i>p</i> > 0.05). The emulsifying stability and foam capacity were significantly lower in protein hydrolysates than protein concentrate (<i>p</i> < 0.05). The antioxidant activity of protein hydrolysates from protein concentrate and Alcalase was higher than that with papain and pepsin (<i>p</i> < 0.05), owing to the higher hydrophobic amino acid content. Raman spectroscopy indicated structural changes in protein α-helices and β-sheets after enzymatic hydrolysis.https://www.mdpi.com/2075-4450/11/12/876insect proteinsblack soldier flyenzymatic hydrolysisfunctional propertiesantioxidant activityamino acids composition |
| spellingShingle | Inayat Batish Devon Brits Pedro Valencia Caio Miyai Shamil Rafeeq Yixiang Xu Magdalini Galanopoulos Edward Sismour Reza Ovissipour Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) Protein Insects insect proteins black soldier fly enzymatic hydrolysis functional properties antioxidant activity amino acids composition |
| title | Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) Protein |
| title_full | Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) Protein |
| title_fullStr | Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) Protein |
| title_full_unstemmed | Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) Protein |
| title_short | Effects of Enzymatic Hydrolysis on the Functional Properties, Antioxidant Activity and Protein Structure of Black Soldier Fly (<i>Hermetia illucens</i>) Protein |
| title_sort | effects of enzymatic hydrolysis on the functional properties antioxidant activity and protein structure of black soldier fly i hermetia illucens i protein |
| topic | insect proteins black soldier fly enzymatic hydrolysis functional properties antioxidant activity amino acids composition |
| url | https://www.mdpi.com/2075-4450/11/12/876 |
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