Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy
The main reserve polysaccharide of plants—starch—is undoubtedly important for humans. One of the main sources of starch is the potato tuber, which is able to preserve starch for a long time during the so-called dormancy period. However, accumulated data show that this dormancy is only relative, whic...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/9/8397 |
| _version_ | 1797602408827715584 |
|---|---|
| author | Lyubov A. Wasserman Oksana O. Kolachevskaya Alexey V. Krivandin Anna G. Filatova Oleg V. Gradov Irina G. Plashchina Georgy A. Romanov |
| author_facet | Lyubov A. Wasserman Oksana O. Kolachevskaya Alexey V. Krivandin Anna G. Filatova Oleg V. Gradov Irina G. Plashchina Georgy A. Romanov |
| author_sort | Lyubov A. Wasserman |
| collection | DOAJ |
| description | The main reserve polysaccharide of plants—starch—is undoubtedly important for humans. One of the main sources of starch is the potato tuber, which is able to preserve starch for a long time during the so-called dormancy period. However, accumulated data show that this dormancy is only relative, which raises the question of the possibility of some kind of starch restructuring during dormancy periods. Here, the effect of long-term periods of tuber rest (at 2–4 °C) on main parameters of starches of potato tubers grown in vivo or in vitro were studied. Along with non-transgenic potatoes, <i>Arabidopsis phytochrome B</i> (<i>AtPHYB</i>) transformants were investigated. Distinct changes in starch micro and macro structures—an increase in proportion of amorphous lamellae and of large-sized and irregular-shaped granules, as well as shifts in thickness of the crystalline lamellae—were detected. The degree of such alterations, more pronounced in <i>AtPHYB</i>-transgenic tubers, increased with the longevity of tuber dormancy. By contrast, the polymorphic crystalline structure (B-type) of starch remained unchanged regardless of dormancy duration. Collectively, our data support the hypothesis that potato starch remains metabolically and structurally labile during the entire tuber life including the dormancy period. The revealed starch remodeling may be considered a process of tuber preadaptation to the upcoming sprouting stage. |
| first_indexed | 2024-03-11T04:16:39Z |
| format | Article |
| id | doaj.art-ea07cbf793254f3a95b46af396c865e8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:16:39Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ea07cbf793254f3a95b46af396c865e82023-11-17T23:09:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-01249839710.3390/ijms24098397Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber DormancyLyubov A. Wasserman0Oksana O. Kolachevskaya1Alexey V. Krivandin2Anna G. Filatova3Oleg V. Gradov4Irina G. Plashchina5Georgy A. Romanov6Emanuel Institute of Biochemical Physics RAS (IBCP RAS), Kosygina Str. 4, 119334 Moscow, RussiaTimiryazev Institute of Plant Physiology RAS (IPP RAS), Botanicheskaya Str. 35, 127276 Moscow, RussiaEmanuel Institute of Biochemical Physics RAS (IBCP RAS), Kosygina Str. 4, 119334 Moscow, RussiaSemenov Federal Research Center for Chemical Physics RAS (ICP RAS), Kosygina Str. 4, 119991 Moscow, RussiaEmanuel Institute of Biochemical Physics RAS (IBCP RAS), Kosygina Str. 4, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics RAS (IBCP RAS), Kosygina Str. 4, 119334 Moscow, RussiaTimiryazev Institute of Plant Physiology RAS (IPP RAS), Botanicheskaya Str. 35, 127276 Moscow, RussiaThe main reserve polysaccharide of plants—starch—is undoubtedly important for humans. One of the main sources of starch is the potato tuber, which is able to preserve starch for a long time during the so-called dormancy period. However, accumulated data show that this dormancy is only relative, which raises the question of the possibility of some kind of starch restructuring during dormancy periods. Here, the effect of long-term periods of tuber rest (at 2–4 °C) on main parameters of starches of potato tubers grown in vivo or in vitro were studied. Along with non-transgenic potatoes, <i>Arabidopsis phytochrome B</i> (<i>AtPHYB</i>) transformants were investigated. Distinct changes in starch micro and macro structures—an increase in proportion of amorphous lamellae and of large-sized and irregular-shaped granules, as well as shifts in thickness of the crystalline lamellae—were detected. The degree of such alterations, more pronounced in <i>AtPHYB</i>-transgenic tubers, increased with the longevity of tuber dormancy. By contrast, the polymorphic crystalline structure (B-type) of starch remained unchanged regardless of dormancy duration. Collectively, our data support the hypothesis that potato starch remains metabolically and structurally labile during the entire tuber life including the dormancy period. The revealed starch remodeling may be considered a process of tuber preadaptation to the upcoming sprouting stage.https://www.mdpi.com/1422-0067/24/9/8397starch<i>Solanum tuberosum</i>tuber dormancytransgenic potato<i>AtPHYB</i> genegranulometric analysis |
| spellingShingle | Lyubov A. Wasserman Oksana O. Kolachevskaya Alexey V. Krivandin Anna G. Filatova Oleg V. Gradov Irina G. Plashchina Georgy A. Romanov Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy International Journal of Molecular Sciences starch <i>Solanum tuberosum</i> tuber dormancy transgenic potato <i>AtPHYB</i> gene granulometric analysis |
| title | Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy |
| title_full | Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy |
| title_fullStr | Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy |
| title_full_unstemmed | Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy |
| title_short | Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy |
| title_sort | changes in structural and thermodynamic properties of starch during potato tuber dormancy |
| topic | starch <i>Solanum tuberosum</i> tuber dormancy transgenic potato <i>AtPHYB</i> gene granulometric analysis |
| url | https://www.mdpi.com/1422-0067/24/9/8397 |
| work_keys_str_mv | AT lyubovawasserman changesinstructuralandthermodynamicpropertiesofstarchduringpotatotuberdormancy AT oksanaokolachevskaya changesinstructuralandthermodynamicpropertiesofstarchduringpotatotuberdormancy AT alexeyvkrivandin changesinstructuralandthermodynamicpropertiesofstarchduringpotatotuberdormancy AT annagfilatova changesinstructuralandthermodynamicpropertiesofstarchduringpotatotuberdormancy AT olegvgradov changesinstructuralandthermodynamicpropertiesofstarchduringpotatotuberdormancy AT irinagplashchina changesinstructuralandthermodynamicpropertiesofstarchduringpotatotuberdormancy AT georgyaromanov changesinstructuralandthermodynamicpropertiesofstarchduringpotatotuberdormancy |