Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties
This study aimed to assess the effects of a heterofermentative microbial inoculant and storage length on fermentation profile, aerobic stability, and nutrient composition in whole-plant sorghum silage (WPSS) from different varieties. Experiment 1, a completely randomized design with a 2 × 3 factoria...
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.660567/full |
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| author | E. Cole Diepersloot Matheus R. Pupo Lucas G. Ghizzi Lucas G. Ghizzi Jessica O. Gusmão Jessica O. Gusmão Celso Heinzen Cody L. McCary Marcelo O. Wallau Luiz F. Ferraretto |
| author_facet | E. Cole Diepersloot Matheus R. Pupo Lucas G. Ghizzi Lucas G. Ghizzi Jessica O. Gusmão Jessica O. Gusmão Celso Heinzen Cody L. McCary Marcelo O. Wallau Luiz F. Ferraretto |
| author_sort | E. Cole Diepersloot |
| collection | DOAJ |
| description | This study aimed to assess the effects of a heterofermentative microbial inoculant and storage length on fermentation profile, aerobic stability, and nutrient composition in whole-plant sorghum silage (WPSS) from different varieties. Experiment 1, a completely randomized design with a 2 × 3 factorial treatment arrangement, evaluated microbial inoculation [CON (50 mL distilled water) or LBLD (Lactobacillus plantarum DSM 21762, L. buchneri DSM 12856, and L. diolivorans DSM 32074; 300,000 CFU/g of fresh forage)] and storage length (14, 28, or 56 d) in forage WPSS. The LBLD silage had lower pH compared to CON, and greater concentrations of succinic acid, ethanol, 1,2-propanediol (1,2-PD), 1-propanol, 2,3-butanediol and total acids. After 56 d, lactic acid concentration was greater for CON, while acetic acid and aerobic stability were greater in LBLD silage. Experiment 2, a completely randomized design with a 2 × 3 factorial treatment arrangement, evaluated effects of microbial inoculation (same as experiment 1) and storage length (14, 28, or 56 d) in WPSS of three varieties [forage sorghum (Mojo Seed, OPAL, Hereford, TX), sorghum-sudangrass (Dyna-gro Seed, Fullgraze II, Loveland, CO, United States), or sweet sorghum (MAFES Foundation Seed Stocks, Dale, MS State, MS)]. The LBLD forage sorghum had greater acetic acid and 1,2-PD concentrations at 56 d and 28 d, respectively, but lower concentrations of propionic acid at 56 d and butyric acid at 14 and 28 d. Additionally, WSC concentration was greater for CON than LBLD at 28 d. Furthermore, CON sweet sorghum had greater lactic acid, propionic acid, and butyric acid concentrations. However, greater acetic acid and 1,2-PD were observed for LBLD sweet sorghum. The CON sweet sorghum had greater concentration of WSC and yeast counts. The CON sorghum sudangrass had greater lactic and butyric acid concentrations than LBLD at 14 d, but lower acetic acid and 1,2-PD concentrations at 56 d. Yeast counts were greater for CON than LBLD sorghum sudangrass silage. Overall, results indicate inoculation of WPSS with Lactobacillus plantarum DSM 21762, L. buchneri DSM 12856, and L. diolivorans DSM 32074 improves heterofermentative co-fermentation allowing the accumulation of acetic acid concentration and increasing antifungal capacities and aerobic stability of WPSS. |
| first_indexed | 2024-12-19T06:51:44Z |
| format | Article |
| id | doaj.art-ea407b5772094b6d89193c760a6a8eed |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T06:51:44Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-ea407b5772094b6d89193c760a6a8eed2022-12-21T20:31:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-04-011210.3389/fmicb.2021.660567660567Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different VarietiesE. Cole Diepersloot0Matheus R. Pupo1Lucas G. Ghizzi2Lucas G. Ghizzi3Jessica O. Gusmão4Jessica O. Gusmão5Celso Heinzen6Cody L. McCary7Marcelo O. Wallau8Luiz F. Ferraretto9Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI, United StatesDepartment of Animal and Dairy Sciences, University of Wisconsin, Madison, WI, United StatesDepartment of Animal Sciences, University of Florida, Gainesville, FL, United StatesDepartment of Animal Nutrition and Animal Production, University of São Paulo, Pirassununga, BrazilDepartment of Animal Sciences, University of Florida, Gainesville, FL, United StatesDepartment of Animal Science, Federal University of Lavras, Minas Gerais, BrazilDepartment of Animal and Dairy Sciences, University of Wisconsin, Madison, WI, United StatesDepartment of Animal Sciences, University of Florida, Gainesville, FL, United StatesAgronomy Department, University of Florida, Gainesville, FL, United StatesDepartment of Animal and Dairy Sciences, University of Wisconsin, Madison, WI, United StatesThis study aimed to assess the effects of a heterofermentative microbial inoculant and storage length on fermentation profile, aerobic stability, and nutrient composition in whole-plant sorghum silage (WPSS) from different varieties. Experiment 1, a completely randomized design with a 2 × 3 factorial treatment arrangement, evaluated microbial inoculation [CON (50 mL distilled water) or LBLD (Lactobacillus plantarum DSM 21762, L. buchneri DSM 12856, and L. diolivorans DSM 32074; 300,000 CFU/g of fresh forage)] and storage length (14, 28, or 56 d) in forage WPSS. The LBLD silage had lower pH compared to CON, and greater concentrations of succinic acid, ethanol, 1,2-propanediol (1,2-PD), 1-propanol, 2,3-butanediol and total acids. After 56 d, lactic acid concentration was greater for CON, while acetic acid and aerobic stability were greater in LBLD silage. Experiment 2, a completely randomized design with a 2 × 3 factorial treatment arrangement, evaluated effects of microbial inoculation (same as experiment 1) and storage length (14, 28, or 56 d) in WPSS of three varieties [forage sorghum (Mojo Seed, OPAL, Hereford, TX), sorghum-sudangrass (Dyna-gro Seed, Fullgraze II, Loveland, CO, United States), or sweet sorghum (MAFES Foundation Seed Stocks, Dale, MS State, MS)]. The LBLD forage sorghum had greater acetic acid and 1,2-PD concentrations at 56 d and 28 d, respectively, but lower concentrations of propionic acid at 56 d and butyric acid at 14 and 28 d. Additionally, WSC concentration was greater for CON than LBLD at 28 d. Furthermore, CON sweet sorghum had greater lactic acid, propionic acid, and butyric acid concentrations. However, greater acetic acid and 1,2-PD were observed for LBLD sweet sorghum. The CON sweet sorghum had greater concentration of WSC and yeast counts. The CON sorghum sudangrass had greater lactic and butyric acid concentrations than LBLD at 14 d, but lower acetic acid and 1,2-PD concentrations at 56 d. Yeast counts were greater for CON than LBLD sorghum sudangrass silage. Overall, results indicate inoculation of WPSS with Lactobacillus plantarum DSM 21762, L. buchneri DSM 12856, and L. diolivorans DSM 32074 improves heterofermentative co-fermentation allowing the accumulation of acetic acid concentration and increasing antifungal capacities and aerobic stability of WPSS.https://www.frontiersin.org/articles/10.3389/fmicb.2021.660567/fullL. buchneriL. diolivoransvarietyaerobic stability12-propanediol. |
| spellingShingle | E. Cole Diepersloot Matheus R. Pupo Lucas G. Ghizzi Lucas G. Ghizzi Jessica O. Gusmão Jessica O. Gusmão Celso Heinzen Cody L. McCary Marcelo O. Wallau Luiz F. Ferraretto Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties Frontiers in Microbiology L. buchneri L. diolivorans variety aerobic stability 1 2-propanediol. |
| title | Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties |
| title_full | Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties |
| title_fullStr | Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties |
| title_full_unstemmed | Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties |
| title_short | Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties |
| title_sort | effects of microbial inoculation and storage length on fermentation profile and nutrient composition of whole plant sorghum silage of different varieties |
| topic | L. buchneri L. diolivorans variety aerobic stability 1 2-propanediol. |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.660567/full |
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