Identification and functional comparison of novel alternatively spliced isoforms of human YAP
As a key effector of the Hippo pathway, yes‐associated protein (YAP) is a major regulator of cell proliferation and apoptosis. In this study, 23 hYAP isoforms were identified in HEK293 cells, with 14 isoforms being reported for the first time. These isoforms were classified into hYAP‐a and hYAP‐b is...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13618 |
| _version_ | 1797810959804268544 |
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| author | Lianlian Liu Junlei Zhang Jiaqi Wang Yanping Tian Jiali Wang Yixiao Xu Yuda Cheng Meng Yu Jiangjun Wang Yi Yang Xueyue Wang Ran Yang Wei Wu Chen Zhang Yan Hu Rui Jian Lan Xiao Yan Ruan |
| author_facet | Lianlian Liu Junlei Zhang Jiaqi Wang Yanping Tian Jiali Wang Yixiao Xu Yuda Cheng Meng Yu Jiangjun Wang Yi Yang Xueyue Wang Ran Yang Wei Wu Chen Zhang Yan Hu Rui Jian Lan Xiao Yan Ruan |
| author_sort | Lianlian Liu |
| collection | DOAJ |
| description | As a key effector of the Hippo pathway, yes‐associated protein (YAP) is a major regulator of cell proliferation and apoptosis. In this study, 23 hYAP isoforms were identified in HEK293 cells, with 14 isoforms being reported for the first time. These isoforms were classified into hYAP‐a and hYAP‐b isoforms based on the variation in exon 1. The two groups of isoforms showed distinctly different subcellular localizations. hYAP‐a isoforms could activate TEAD‐ or P73‐mediated transcription, affect the proliferation rate, and enhance the cellular chemosensitivity of HEK293 cells. Moreover, different activation abilities and pro‐cytotoxic effects were observed among hYAP‐a isoforms. However, hYAP‐b isoforms were not found to exert any significant biological effects. Our findings add to the knowledge of YAP gene structure and protein‐coding capacity and will help in the elucidation of the function and related molecular mechanisms of the Hippo‐YAP signaling pathway. |
| first_indexed | 2024-03-13T07:15:31Z |
| format | Article |
| id | doaj.art-ea4aca82387e4a34b1874e5803cd1bc3 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-13T07:15:31Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-ea4aca82387e4a34b1874e5803cd1bc32023-06-05T08:21:04ZengWileyFEBS Open Bio2211-54632023-06-011361001101410.1002/2211-5463.13618Identification and functional comparison of novel alternatively spliced isoforms of human YAPLianlian Liu0Junlei Zhang1Jiaqi Wang2Yanping Tian3Jiali Wang4Yixiao Xu5Yuda Cheng6Meng Yu7Jiangjun Wang8Yi Yang9Xueyue Wang10Ran Yang11Wei Wu12Chen Zhang13Yan Hu14Rui Jian15Lan Xiao16Yan Ruan17Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaExperimental Center of Basic Medicine, College of Basic Medical Sciences Army Medical University Chongqing ChinaDepartment of Pediatrics The General Hospital of PLA Tibet Military Area Command Lhasa ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaThoracic Surgery Department Southwest Hospital, The First Hospital Affiliated to Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaDepartment of Military Basic Training and Army Management, Army Health Service Training Base Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaDepartment of Histology and Embryology, Chongqing Key Laboratory of Neurobiology, Brain and Intelligence Research Key Laboratory of Chongqing Education Commission Army Medical University Chongqing ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing ChinaAs a key effector of the Hippo pathway, yes‐associated protein (YAP) is a major regulator of cell proliferation and apoptosis. In this study, 23 hYAP isoforms were identified in HEK293 cells, with 14 isoforms being reported for the first time. These isoforms were classified into hYAP‐a and hYAP‐b isoforms based on the variation in exon 1. The two groups of isoforms showed distinctly different subcellular localizations. hYAP‐a isoforms could activate TEAD‐ or P73‐mediated transcription, affect the proliferation rate, and enhance the cellular chemosensitivity of HEK293 cells. Moreover, different activation abilities and pro‐cytotoxic effects were observed among hYAP‐a isoforms. However, hYAP‐b isoforms were not found to exert any significant biological effects. Our findings add to the knowledge of YAP gene structure and protein‐coding capacity and will help in the elucidation of the function and related molecular mechanisms of the Hippo‐YAP signaling pathway.https://doi.org/10.1002/2211-5463.13618alternative splicingcell chemosensitivitytranscriptional activation abilityYAP |
| spellingShingle | Lianlian Liu Junlei Zhang Jiaqi Wang Yanping Tian Jiali Wang Yixiao Xu Yuda Cheng Meng Yu Jiangjun Wang Yi Yang Xueyue Wang Ran Yang Wei Wu Chen Zhang Yan Hu Rui Jian Lan Xiao Yan Ruan Identification and functional comparison of novel alternatively spliced isoforms of human YAP FEBS Open Bio alternative splicing cell chemosensitivity transcriptional activation ability YAP |
| title | Identification and functional comparison of novel alternatively spliced isoforms of human YAP |
| title_full | Identification and functional comparison of novel alternatively spliced isoforms of human YAP |
| title_fullStr | Identification and functional comparison of novel alternatively spliced isoforms of human YAP |
| title_full_unstemmed | Identification and functional comparison of novel alternatively spliced isoforms of human YAP |
| title_short | Identification and functional comparison of novel alternatively spliced isoforms of human YAP |
| title_sort | identification and functional comparison of novel alternatively spliced isoforms of human yap |
| topic | alternative splicing cell chemosensitivity transcriptional activation ability YAP |
| url | https://doi.org/10.1002/2211-5463.13618 |
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