A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cells
A combination of ethanolic extract of Phyllanthus emblica fruits and ethanolic extract of Alpinia galanga rhizomes at a ratio of 7:3 (PE–AE (7:3)) exhibited synergistic antioxidant activities against DPPH·, ABTS·+, and superoxide anion (O2·−) radicals with Combination Indices (CIs) of 0.692 ± 0.048,...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618300239 |
| _version_ | 1818564239166537728 |
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| author | Chaisak Chansriniyom Phichaporn Bunwatcharaphansakun Waleewan Eaknai Nonthaneth Nalinratana Arkornnut Ratanawong Mattaka Khongkow Rataya Luechapudiporn |
| author_facet | Chaisak Chansriniyom Phichaporn Bunwatcharaphansakun Waleewan Eaknai Nonthaneth Nalinratana Arkornnut Ratanawong Mattaka Khongkow Rataya Luechapudiporn |
| author_sort | Chaisak Chansriniyom |
| collection | DOAJ |
| description | A combination of ethanolic extract of Phyllanthus emblica fruits and ethanolic extract of Alpinia galanga rhizomes at a ratio of 7:3 (PE–AE (7:3)) exhibited synergistic antioxidant activities against DPPH·, ABTS·+, and superoxide anion (O2·−) radicals with Combination Indices (CIs) of 0.692 ± 0.048, 0.802 ± 0.033, and 0.840 ± 0.023, respectively. At concentrations of 20 and 50 µg/mL, the PE–AE (7:3) showed a significant decrease in intracellular reactive oxygen species against H2O2-induced oxidative stress in an endothelial cell line (ECV304) in dose-dependent manner, as well as an increase in cell survival. In addition, The PE–AE (7:3) at 50 µg/mL was as potent as quercetin at 10 µg/mL in the inhibition of H2O2-induced lipid peroxidation. The combinations of ellagic acid and hydroxycinnamic derivatives which attributed to synergistic antiradicals enhanced cytoprotective activity. This first report on synergistic combination of PE and AE has the potential to develop as new nutritional antioxidants. |
| first_indexed | 2024-12-14T01:26:32Z |
| format | Article |
| id | doaj.art-eab1e7f8911047b6aec56f7190921a74 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T01:26:32Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-eab1e7f8911047b6aec56f7190921a742022-12-21T23:22:11ZengElsevierJournal of Functional Foods1756-46462018-04-01434454A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cellsChaisak Chansriniyom0Phichaporn Bunwatcharaphansakun1Waleewan Eaknai2Nonthaneth Nalinratana3Arkornnut Ratanawong4Mattaka Khongkow5Rataya Luechapudiporn6NANOTEC, National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, Bangkok 10330, ThailandNANOTEC, National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, ThailandNANOTEC, National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, ThailandDepartment of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, Bangkok 10330, ThailandInterdisciplinary Program in Pharmacology, Graduate School, Chulalongkorn University, Pathumwan, Bangkok 10330, ThailandNANOTEC, National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, ThailandDepartment of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; Corresponding author.A combination of ethanolic extract of Phyllanthus emblica fruits and ethanolic extract of Alpinia galanga rhizomes at a ratio of 7:3 (PE–AE (7:3)) exhibited synergistic antioxidant activities against DPPH·, ABTS·+, and superoxide anion (O2·−) radicals with Combination Indices (CIs) of 0.692 ± 0.048, 0.802 ± 0.033, and 0.840 ± 0.023, respectively. At concentrations of 20 and 50 µg/mL, the PE–AE (7:3) showed a significant decrease in intracellular reactive oxygen species against H2O2-induced oxidative stress in an endothelial cell line (ECV304) in dose-dependent manner, as well as an increase in cell survival. In addition, The PE–AE (7:3) at 50 µg/mL was as potent as quercetin at 10 µg/mL in the inhibition of H2O2-induced lipid peroxidation. The combinations of ellagic acid and hydroxycinnamic derivatives which attributed to synergistic antiradicals enhanced cytoprotective activity. This first report on synergistic combination of PE and AE has the potential to develop as new nutritional antioxidants.http://www.sciencedirect.com/science/article/pii/S1756464618300239Phyllanthus emblicaAlpinia galangaSynergistic effectsAntioxidationLipid peroxidationhuman ECV304 cells |
| spellingShingle | Chaisak Chansriniyom Phichaporn Bunwatcharaphansakun Waleewan Eaknai Nonthaneth Nalinratana Arkornnut Ratanawong Mattaka Khongkow Rataya Luechapudiporn A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cells Journal of Functional Foods Phyllanthus emblica Alpinia galanga Synergistic effects Antioxidation Lipid peroxidation human ECV304 cells |
| title | A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cells |
| title_full | A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cells |
| title_fullStr | A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cells |
| title_full_unstemmed | A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cells |
| title_short | A synergistic combination of Phyllanthus emblica and Alpinia galanga against H2O2-induced oxidative stress and lipid peroxidation in human ECV304 cells |
| title_sort | synergistic combination of phyllanthus emblica and alpinia galanga against h2o2 induced oxidative stress and lipid peroxidation in human ecv304 cells |
| topic | Phyllanthus emblica Alpinia galanga Synergistic effects Antioxidation Lipid peroxidation human ECV304 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618300239 |
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