The effect of rewarming media composition on the ammonia detoxification ability of cold preserved rat hepatocytes

We examined how different media composition of rewarming solutions affected ammonium detoxification function, urea synthesis and the viability of hepatocytes after 72 hs of cold storage in UW solution. Freshly isolated rat hepatocytes were incubated at 37 ºC in a cell culture medium (MEM-E) with 3 m...

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Bibliographic Details
Main Authors: Sebastián Dante Calligaris, Edgardo Elvio Guibert, Joaquín Valentín Rodríguez
Format: Article
Language:English
Published: Elsevier 2002-07-01
Series:Annals of Hepatology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1665268119321738