Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR
Due to the genetic mutation (fa) in the gene encoding for leptin receptor, homozygous Zucker rats (fa−/−) develop excessive adiposity and become an experimental animal model in obesity and metabolic-related diseases research. Based on tetra-primer amplification refractory mutation system-polymerase...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402307367X |
| _version_ | 1827803054701805568 |
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| author | Xinyun Xu Xinge Hu Guodong Ma Tiannan Wang Jayne Wu Xiaojuan Zhu Guoxun Chen Ling Zhao Jiangang Chen |
| author_facet | Xinyun Xu Xinge Hu Guodong Ma Tiannan Wang Jayne Wu Xiaojuan Zhu Guoxun Chen Ling Zhao Jiangang Chen |
| author_sort | Xinyun Xu |
| collection | DOAJ |
| description | Due to the genetic mutation (fa) in the gene encoding for leptin receptor, homozygous Zucker rats (fa−/−) develop excessive adiposity and become an experimental animal model in obesity and metabolic-related diseases research. Based on tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), we developed a method to quickly genotype Zucker rats with a mutated fa allele from their wildtype littermates. The three genotypes are clearly discriminated on 2.0% agarose gel. Our method can be used as a reliable tool to set up and maintain the breeding colony in animal facilities as well as assign animals to control and treatment groups based on their genotypes for animal studies. |
| first_indexed | 2024-03-11T20:48:54Z |
| format | Article |
| id | doaj.art-eae30d21e4e441f5b2dd0852dcfc8ae8 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:48:54Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-eae30d21e4e441f5b2dd0852dcfc8ae82023-10-01T06:02:44ZengElsevierHeliyon2405-84402023-09-0199e20159Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCRXinyun Xu0Xinge Hu1Guodong Ma2Tiannan Wang3Jayne Wu4Xiaojuan Zhu5Guoxun Chen6Ling Zhao7Jiangang Chen8Department of Nutrition, The University of Tennessee Knoxville, TN, 37996, United StatesDepartment of Nutrition, The University of Tennessee Knoxville, TN, 37996, United StatesDepartment of Nutrition, The University of Tennessee Knoxville, TN, 37996, United StatesDepartment of Nutrition, The University of Tennessee Knoxville, TN, 37996, United StatesDepartment of Electrical Engineering and Computer Science, The University of Tennessee Knoxville, TN, 37996, United StatesOffice of Information Technology, The University of Tennessee Knoxville, TN, 37996, United StatesDepartment of Nutrition, The University of Tennessee Knoxville, TN, 37996, United States; Corresponding author.Department of Nutrition, The University of Tennessee Knoxville, TN, 37996, United States; Corresponding author.Department of Public Health, The University of Tennessee Knoxville, TN, 37996, United States; Corresponding author.Due to the genetic mutation (fa) in the gene encoding for leptin receptor, homozygous Zucker rats (fa−/−) develop excessive adiposity and become an experimental animal model in obesity and metabolic-related diseases research. Based on tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), we developed a method to quickly genotype Zucker rats with a mutated fa allele from their wildtype littermates. The three genotypes are clearly discriminated on 2.0% agarose gel. Our method can be used as a reliable tool to set up and maintain the breeding colony in animal facilities as well as assign animals to control and treatment groups based on their genotypes for animal studies.http://www.sciencedirect.com/science/article/pii/S240584402307367Xfa mutationLeptin receptorTetra-primer amplification-refractory mutation system (ARMS)Zucker ratsObesity |
| spellingShingle | Xinyun Xu Xinge Hu Guodong Ma Tiannan Wang Jayne Wu Xiaojuan Zhu Guoxun Chen Ling Zhao Jiangang Chen Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR Heliyon fa mutation Leptin receptor Tetra-primer amplification-refractory mutation system (ARMS) Zucker rats Obesity |
| title | Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR |
| title_full | Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR |
| title_fullStr | Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR |
| title_full_unstemmed | Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR |
| title_short | Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR |
| title_sort | detecting fa leptin receptor mutation in zucker rats with tetra primer amplification refractory mutation system arms pcr |
| topic | fa mutation Leptin receptor Tetra-primer amplification-refractory mutation system (ARMS) Zucker rats Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S240584402307367X |
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