Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration.
Cholera toxin (CT) is a mucosal adjuvant capable of inducing strong immune responses to co-administered antigens following oral or intranasal immunization of mice. To date, the direct effect of CT on antigen-specific CD4(+) T cell migration and proliferation profiles in vivo is not well characterize...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3609821?pdf=render |
| _version_ | 1829101415366131712 |
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| author | Maria Kaparakis-Liaskos Michelle D Tate Jason D Price Martin Pearse Odilia L C Wijburg |
| author_facet | Maria Kaparakis-Liaskos Michelle D Tate Jason D Price Martin Pearse Odilia L C Wijburg |
| author_sort | Maria Kaparakis-Liaskos |
| collection | DOAJ |
| description | Cholera toxin (CT) is a mucosal adjuvant capable of inducing strong immune responses to co-administered antigens following oral or intranasal immunization of mice. To date, the direct effect of CT on antigen-specific CD4(+) T cell migration and proliferation profiles in vivo is not well characterized. In this study, the effect of CT on the migration pattern and proliferative responses of adoptively transferred, CD4(+) TCR transgenic T cells in orally or intranasally vaccinated mice, was analyzed by flow cytometry. GFP-expressing or CFSE-labeled OT-II lymphocytes were adoptively transferred to naïve C57BL/6 mice, and mice were subsequently vaccinated with OVA with or without CT via the oral or intranasal route. CT did not alter the migration pattern of antigen-specific T cells, regardless of the route of immunization, but increased the number of transgenic CD4(+) T cells in draining lymphoid tissue. This increase in the number of transgenic CD4(+) T cells was not due to cells undergoing more rounds of cellular division in vivo, suggesting that CT may exert an indirect adjuvant effect on CD4(+) T cells. The findings reported here suggest that CT functions as a mucosal adjuvant by increasing the number of antigen specific CD4(+) T cells independent of their migration pattern or kinetics of cellular division. |
| first_indexed | 2024-12-10T22:46:32Z |
| format | Article |
| id | doaj.art-eaf836a766a94207bbc018145ba640eb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:46:32Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-eaf836a766a94207bbc018145ba640eb2022-12-22T01:30:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5993410.1371/journal.pone.0059934Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration.Maria Kaparakis-LiaskosMichelle D TateJason D PriceMartin PearseOdilia L C WijburgCholera toxin (CT) is a mucosal adjuvant capable of inducing strong immune responses to co-administered antigens following oral or intranasal immunization of mice. To date, the direct effect of CT on antigen-specific CD4(+) T cell migration and proliferation profiles in vivo is not well characterized. In this study, the effect of CT on the migration pattern and proliferative responses of adoptively transferred, CD4(+) TCR transgenic T cells in orally or intranasally vaccinated mice, was analyzed by flow cytometry. GFP-expressing or CFSE-labeled OT-II lymphocytes were adoptively transferred to naïve C57BL/6 mice, and mice were subsequently vaccinated with OVA with or without CT via the oral or intranasal route. CT did not alter the migration pattern of antigen-specific T cells, regardless of the route of immunization, but increased the number of transgenic CD4(+) T cells in draining lymphoid tissue. This increase in the number of transgenic CD4(+) T cells was not due to cells undergoing more rounds of cellular division in vivo, suggesting that CT may exert an indirect adjuvant effect on CD4(+) T cells. The findings reported here suggest that CT functions as a mucosal adjuvant by increasing the number of antigen specific CD4(+) T cells independent of their migration pattern or kinetics of cellular division.http://europepmc.org/articles/PMC3609821?pdf=render |
| spellingShingle | Maria Kaparakis-Liaskos Michelle D Tate Jason D Price Martin Pearse Odilia L C Wijburg Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration. PLoS ONE |
| title | Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration. |
| title_full | Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration. |
| title_fullStr | Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration. |
| title_full_unstemmed | Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration. |
| title_short | Increased antigen specific T cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration. |
| title_sort | increased antigen specific t cell numbers in the absence of altered migration or division rates as a result of mucosal cholera toxin administration |
| url | http://europepmc.org/articles/PMC3609821?pdf=render |
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