A Uniform Benchmark for Testing SsrA-Derived Degrons in the <i>Escherichia coli</i> ClpXP Degradation Pathway

The ssrA degron is commonly used in fusion proteins to control protein stability in bacteria or as an interaction module. These applications often rely on the modular activities of the ssrA tag in binding to the SspB adaptor and in engaging the ClpXP protease. However, a comparison of these activiti...

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Bibliographic Details
Main Authors: Maria Magdalena Klimecka, Anna Antosiewicz, Matylda Anna Izert, Patrycja Emanuela Szybowska, Piotr Krzysztof Twardowski, Clara Delaunay, Maria Wiktoria Górna
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/19/5936