Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract
Red ginseng has powerful potential for use as a prebiotic, but its use is limited due to its antibacterial activity. The aim of this study is to present <i>panax ginseng</i>’s endophytic lactic acid bacteria capable to overcome the antibacterial activity of red ginseng and improve their...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/7/4/238 |
| _version_ | 1827672603917025280 |
|---|---|
| author | Sung-Joon Mo Bora Nam Chu-Hyun Bae Soo-Dong Park Jae-Jung Shim Jung-Lyoul Lee |
| author_facet | Sung-Joon Mo Bora Nam Chu-Hyun Bae Soo-Dong Park Jae-Jung Shim Jung-Lyoul Lee |
| author_sort | Sung-Joon Mo |
| collection | DOAJ |
| description | Red ginseng has powerful potential for use as a prebiotic, but its use is limited due to its antibacterial activity. The aim of this study is to present <i>panax ginseng</i>’s endophytic lactic acid bacteria capable to overcome the antibacterial activity of red ginseng and improve their characteristic. <i>Lactobacillus paracasei</i> HY7017 (HY7017) was cultured in a medium supplemented with red ginseng. The probiotic properties and immune-enhancing effects of HY7017 were investigated in vitro and in vivo. HY7017 was proliferated strongly in RGE and had significantly improved properties compared with an <i>L. paracasei</i> type strain ATCC25302. HY7017 cultured in RGE-supplemented medium increased the production of nitric oxide, TNF-α, and IL-6 in macrophages, and increased IL-12 and IFN-γ secretion in splenocytes. Furthermore, HY7017 restored WBC counts, increased the amount of IL-2 and IFN-γ released, and enhanced the cytotoxicity of natural killer cells when orally administered to immunosuppressed mice. Moreover, HY7017 has properties that make it suitable as a probiotic, such as stability in the gastrointestinal tract and adhesion to Caco-2 cells. This study showed that HY7017 cultured with RGE may contribute to the development of probiotics to enhance immunity. |
| first_indexed | 2024-03-10T04:10:37Z |
| format | Article |
| id | doaj.art-eb72ce11da584963ad43ef2baac09a35 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T04:10:37Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-eb72ce11da584963ad43ef2baac09a352023-11-23T08:12:51ZengMDPI AGFermentation2311-56372021-10-017423810.3390/fermentation7040238Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng ExtractSung-Joon Mo0Bora Nam1Chu-Hyun Bae2Soo-Dong Park3Jae-Jung Shim4Jung-Lyoul Lee5R & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaRed ginseng has powerful potential for use as a prebiotic, but its use is limited due to its antibacterial activity. The aim of this study is to present <i>panax ginseng</i>’s endophytic lactic acid bacteria capable to overcome the antibacterial activity of red ginseng and improve their characteristic. <i>Lactobacillus paracasei</i> HY7017 (HY7017) was cultured in a medium supplemented with red ginseng. The probiotic properties and immune-enhancing effects of HY7017 were investigated in vitro and in vivo. HY7017 was proliferated strongly in RGE and had significantly improved properties compared with an <i>L. paracasei</i> type strain ATCC25302. HY7017 cultured in RGE-supplemented medium increased the production of nitric oxide, TNF-α, and IL-6 in macrophages, and increased IL-12 and IFN-γ secretion in splenocytes. Furthermore, HY7017 restored WBC counts, increased the amount of IL-2 and IFN-γ released, and enhanced the cytotoxicity of natural killer cells when orally administered to immunosuppressed mice. Moreover, HY7017 has properties that make it suitable as a probiotic, such as stability in the gastrointestinal tract and adhesion to Caco-2 cells. This study showed that HY7017 cultured with RGE may contribute to the development of probiotics to enhance immunity.https://www.mdpi.com/2311-5637/7/4/238immune<i>Lactobacillus paracasei</i>probioticsred ginseng |
| spellingShingle | Sung-Joon Mo Bora Nam Chu-Hyun Bae Soo-Dong Park Jae-Jung Shim Jung-Lyoul Lee Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract Fermentation immune <i>Lactobacillus paracasei</i> probiotics red ginseng |
| title | Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract |
| title_full | Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract |
| title_fullStr | Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract |
| title_full_unstemmed | Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract |
| title_short | Characterization of Novel <i>Lactobacillus paracasei</i> HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract |
| title_sort | characterization of novel i lactobacillus paracasei i hy7017 capable of improving physiological properties and immune enhancing effects using red ginseng extract |
| topic | immune <i>Lactobacillus paracasei</i> probiotics red ginseng |
| url | https://www.mdpi.com/2311-5637/7/4/238 |
| work_keys_str_mv | AT sungjoonmo characterizationofnovelilactobacillusparacaseiihy7017capableofimprovingphysiologicalpropertiesandimmuneenhancingeffectsusingredginsengextract AT boranam characterizationofnovelilactobacillusparacaseiihy7017capableofimprovingphysiologicalpropertiesandimmuneenhancingeffectsusingredginsengextract AT chuhyunbae characterizationofnovelilactobacillusparacaseiihy7017capableofimprovingphysiologicalpropertiesandimmuneenhancingeffectsusingredginsengextract AT soodongpark characterizationofnovelilactobacillusparacaseiihy7017capableofimprovingphysiologicalpropertiesandimmuneenhancingeffectsusingredginsengextract AT jaejungshim characterizationofnovelilactobacillusparacaseiihy7017capableofimprovingphysiologicalpropertiesandimmuneenhancingeffectsusingredginsengextract AT junglyoullee characterizationofnovelilactobacillusparacaseiihy7017capableofimprovingphysiologicalpropertiesandimmuneenhancingeffectsusingredginsengextract |