Selection and Optimization of High-Yielding DNA Isolation Protocol for Quantitative Analyses of Methanogenic Archaea

Selection and Optimization of High-Yielding DNA Isolation Protocol for Quantitative Analyses of Methanogenic Archaea

Methanogenic archaea are a functionally important component of the intestinal microbiota of humans and animals, participating in the utilization of detrimental hydrogen produced during gut fermentation. Despite this, archaeal DNA has rarely been found in intestinal microbiome analyses, which prompts...

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Bibliographic Details
Main Authors: Agata Anna Cisek, Iwona Bąk, Ilona Stefańska, Marian Binek
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Microorganisms
Subjects:
archaea
bead-beating
DNA isolation
gut microbiota
<i>mcrA</i> gene
methanogens
Online Access:https://www.mdpi.com/2076-2607/10/3/523

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https://www.mdpi.com/2076-2607/10/3/523

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