A Detailed Protocol for Constructing a Human Single-Chain Variable Fragment (scFv) Library and Downstream Screening via Phage Display

The development of monoclonal antibodies (mAbs) represents a significant milestone in both basic research and clinical applications due to their target specificity and versatility in therapeutic and diagnostic applications. The innovative strategy of mAb screening, utilizing phage display, facilitat...

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Bibliographic Details
Main Authors: Ziyi Liu, Dokyun Kim, Seokmin Kang, Jae U. Jung
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/7/1/13