Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuria
We employed a Sendai virus-based reprogramming method to transform human lymphoblastoid cell lines (LCL) derived from two individuals diagnosed with phenylketonuria (PKU) into induced pluripotent stem cells (iPSC). This reprogramming process involved the expression of the four Yamanaka factors: KLF4...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124001053 |
| _version_ | 1797235641433456640 |
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| author | Desi Veleva Merve Ay Dmitry A. Ovchinnikov Andrew B.J. Prowse Minal J. Menezes Michael Nafisinia |
| author_facet | Desi Veleva Merve Ay Dmitry A. Ovchinnikov Andrew B.J. Prowse Minal J. Menezes Michael Nafisinia |
| author_sort | Desi Veleva |
| collection | DOAJ |
| description | We employed a Sendai virus-based reprogramming method to transform human lymphoblastoid cell lines (LCL) derived from two individuals diagnosed with phenylketonuria (PKU) into induced pluripotent stem cells (iPSC). This reprogramming process involved the expression of the four Yamanaka factors: KLF4, OCT4, SOX2, and C-MYC. The resulting patient-specific iPSCs exhibited a normal karyotype and expressed endogenous pluripotent markers NANOG and OCT-4. Notably, these iPSCs demonstrated strong differentiation capabilities, giving rise to cell populations representing the ectoderm, endoderm, and mesoderm germ layers. |
| first_indexed | 2024-04-24T16:51:11Z |
| format | Article |
| id | doaj.art-eb9b212926cf4db8ad4b0872f9a31338 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-24T16:51:11Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-eb9b212926cf4db8ad4b0872f9a313382024-03-29T05:49:44ZengElsevierStem Cell Research1873-50612024-06-0177103407Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuriaDesi Veleva0Merve Ay1Dmitry A. Ovchinnikov2Andrew B.J. Prowse3Minal J. Menezes4Michael Nafisinia5StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, AustraliaStemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, AustraliaThe Florey Melbourne Brain Centre/Kenneth Myer Building, The University of Melbourne, 30 Royal Parade, Melbourne VIC 3010 AustraliaStemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, AustraliaDiscipline of Genetic Medicine, Sydney Medical School, University of Sydney, Sydney, NSW, AustraliaDiscipline of Genetic Medicine, Sydney Medical School, University of Sydney, Sydney, NSW, Australia; Storr Liver Centre, Westmead Institute for Medical Research,Sydney, NSW, Australia; Corresponding author at: Storr Liver Centre, Westmead Institute for Medical Research, 176 Hawkesbury Rd, Westmead, NSW 2145, Australia.We employed a Sendai virus-based reprogramming method to transform human lymphoblastoid cell lines (LCL) derived from two individuals diagnosed with phenylketonuria (PKU) into induced pluripotent stem cells (iPSC). This reprogramming process involved the expression of the four Yamanaka factors: KLF4, OCT4, SOX2, and C-MYC. The resulting patient-specific iPSCs exhibited a normal karyotype and expressed endogenous pluripotent markers NANOG and OCT-4. Notably, these iPSCs demonstrated strong differentiation capabilities, giving rise to cell populations representing the ectoderm, endoderm, and mesoderm germ layers.http://www.sciencedirect.com/science/article/pii/S1873506124001053PhenylketonuriaPKUHuman Induced pluripotent stem cells (hiPSC)High throughput drug screeningPersonalised medicineDrug discovery |
| spellingShingle | Desi Veleva Merve Ay Dmitry A. Ovchinnikov Andrew B.J. Prowse Minal J. Menezes Michael Nafisinia Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuria Stem Cell Research Phenylketonuria PKU Human Induced pluripotent stem cells (hiPSC) High throughput drug screening Personalised medicine Drug discovery |
| title | Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuria |
| title_full | Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuria |
| title_fullStr | Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuria |
| title_full_unstemmed | Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuria |
| title_short | Generation of two lymphoblastoid-derived induced pluripotent stem cell (iPSC) lines from patients with phenylketonuria |
| title_sort | generation of two lymphoblastoid derived induced pluripotent stem cell ipsc lines from patients with phenylketonuria |
| topic | Phenylketonuria PKU Human Induced pluripotent stem cells (hiPSC) High throughput drug screening Personalised medicine Drug discovery |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124001053 |
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