Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

Abstract Background Bacillus subtilis, an important industrial microorganism, is commonly used in the production of industrial enzymes. Genome modification is often necessary to improve the production performance of cell. The dual-plasmid CRISPR-Cas9 system suitable for iterative genome editing has...

Full description

Bibliographic Details
Main Authors: Yu Zou, Lu Qiu, Aowen Xie, Wenyuan Han, Shangbo Zhang, Jinshan Li, Shumiao Zhao, Yingjun Li, Yunxiang Liang, Yongmei Hu
Format: Article
Language:English
Published: BMC 2022-08-01
Series:Microbial Cell Factories
Subjects:
Bacillus subtilis
PAMPL
All-in-one system
Iterative genome editing
Douchi fibrinolytic enzyme
Online Access:https://doi.org/10.1186/s12934-022-01896-0

Internet

https://doi.org/10.1186/s12934-022-01896-0

Similar Items