Portable rapid detection of maize chlorotic mottle virus using RT-RAA/CRISPR-Cas12a based lateral flow assay

Portable rapid detection of maize chlorotic mottle virus using RT-RAA/CRISPR-Cas12a based lateral flow assay

IntroductionMaize lethal necrosis seriously threatens maize production worldwide, which was caused by coinfection by maize chlorotic mottle virus (MCMV) and a potyvirid. To effectively control maize lethal necrosis, it is vital to develop a rapid, sensitive, and specific detection method for the ear...

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Bibliographic Details
Main Authors: Rong Lei, Ruirui Kuang, Xuanzi Peng, Zhiyuan Jiao, Zhenxing Zhao, Haolong Cong, Zaifeng Fan, Yongjiang Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Plant Science
Subjects:
MCMV
Cas12a
RT-RAA
one-tube
lateral flow assay
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2023.1088544/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2023.1088544/full

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