The function of tcf3 in medaka embryos: efficient knockdown with pePNAs
Abstract Background The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we teste...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12896-017-0411-0 |
| _version_ | 1831837619394183168 |
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| author | Gerlinde Doenz Sebastian Dorn Narges Aghaallaei Baubak Bajoghli Elisabeth Riegel Michaela Aigner Holger Bock Birgit Werner Thomas Lindhorst Thomas Czerny |
| author_facet | Gerlinde Doenz Sebastian Dorn Narges Aghaallaei Baubak Bajoghli Elisabeth Riegel Michaela Aigner Holger Bock Birgit Werner Thomas Lindhorst Thomas Czerny |
| author_sort | Gerlinde Doenz |
| collection | DOAJ |
| description | Abstract Background The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. Results A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes. Conclusions Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b. In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation. |
| first_indexed | 2024-12-23T05:18:07Z |
| format | Article |
| id | doaj.art-ebcc5c44420f40ddbd3a7bce3da58446 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-23T05:18:07Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-ebcc5c44420f40ddbd3a7bce3da584462022-12-21T17:58:46ZengBMCBMC Biotechnology1472-67502018-01-0118111410.1186/s12896-017-0411-0The function of tcf3 in medaka embryos: efficient knockdown with pePNAsGerlinde Doenz0Sebastian Dorn1Narges Aghaallaei2Baubak Bajoghli3Elisabeth Riegel4Michaela Aigner5Holger Bock6Birgit Werner7Thomas Lindhorst8Thomas Czerny9Department for Applied Life Sciences, University of Applied Sciences, FH Campus WienDepartment for Applied Life Sciences, University of Applied Sciences, FH Campus WienCentre for Organismal Studies (COS), University of HeidelbergDepartment of Hematology, Oncology, Immunology, Rheumatology and Pulmonology, University Hospital TübingenDepartment for Applied Life Sciences, University of Applied Sciences, FH Campus WienSandoz GmbHCAST Gründungszentrum GmbHUGISense AG, c/o Nordwind Capital GmbHUGISense AG, c/o Nordwind Capital GmbHDepartment for Applied Life Sciences, University of Applied Sciences, FH Campus WienAbstract Background The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. Results A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes. Conclusions Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b. In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation.http://link.springer.com/article/10.1186/s12896-017-0411-0PNAKnockdownMedakaTcf3GrouchoTle |
| spellingShingle | Gerlinde Doenz Sebastian Dorn Narges Aghaallaei Baubak Bajoghli Elisabeth Riegel Michaela Aigner Holger Bock Birgit Werner Thomas Lindhorst Thomas Czerny The function of tcf3 in medaka embryos: efficient knockdown with pePNAs BMC Biotechnology PNA Knockdown Medaka Tcf3 Groucho Tle |
| title | The function of tcf3 in medaka embryos: efficient knockdown with pePNAs |
| title_full | The function of tcf3 in medaka embryos: efficient knockdown with pePNAs |
| title_fullStr | The function of tcf3 in medaka embryos: efficient knockdown with pePNAs |
| title_full_unstemmed | The function of tcf3 in medaka embryos: efficient knockdown with pePNAs |
| title_short | The function of tcf3 in medaka embryos: efficient knockdown with pePNAs |
| title_sort | function of tcf3 in medaka embryos efficient knockdown with pepnas |
| topic | PNA Knockdown Medaka Tcf3 Groucho Tle |
| url | http://link.springer.com/article/10.1186/s12896-017-0411-0 |
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