Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces

Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces

Cattle are considered to be the main reservoir for Shiga toxin-producing Escherichia coli (STEC) and are often the direct or indirect source of STEC outbreaks in humans. Accurate measurement of the concentration of shed STEC in cattle feces could be a key answer to questions concerning transmission...

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Bibliografski detalji
Glavni autori: Bavo Verhaegen, Koen De Reu, Lieven De Zutter, Karen Verstraete, Marc Heyndrickx, Els Van Coillie
Format: Članak
Jezik:English
Izdano: MDPI AG 2016-05-01
Serija:Toxins
Teme:
Shiga Toxin-producing Escherichia coli
real-time qualitative PCR
droplet digital PCR
PCR inhibition
cattle
Online pristup:http://www.mdpi.com/2072-6651/8/5/157

Internet

http://www.mdpi.com/2072-6651/8/5/157

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