Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

This study investigated the effect of glycerol added in different phases of sperm equilibration on CASA and flow cytometry parameters of thawed ram spermatozoa. Sperm was collected from adult Wallachian rams. The freezing extender was glycerol-free ANDROMED<sup>®</sup> (Minitub GmbH, Tie...

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Bibliographic Details
Main Authors: Anežka Málková, Filipp Georgijevič Savvulidi, Martin Ptáček, Karolína Machová, Martina Janošíková, Szabolcs Nagy, Luděk Stádník
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Animals
Subjects:
cryopreservation capacity
sperm motility
plasma membrane and acrosome intactness
mitochondrial membrane potential
Online Access:https://www.mdpi.com/2076-2615/13/7/1200

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https://www.mdpi.com/2076-2615/13/7/1200

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