Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

This study investigated the effect of glycerol added in different phases of sperm equilibration on CASA and flow cytometry parameters of thawed ram spermatozoa. Sperm was collected from adult Wallachian rams. The freezing extender was glycerol-free ANDROMED<sup>®</sup> (Minitub GmbH, Tie...

Full description

Bibliographic Details
Main Authors:	Anežka Málková, Filipp Georgijevič Savvulidi, Martin Ptáček, Karolína Machová, Martina Janošíková, Szabolcs Nagy, Luděk Stádník
Format:	Article
Language:	English
Published:	MDPI AG 2023-03-01
Series:	Animals
Subjects:	cryopreservation capacity sperm motility plasma membrane and acrosome intactness mitochondrial membrane potential
Online Access:	https://www.mdpi.com/2076-2615/13/7/1200

Similar Items

Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program
by: Filipp Georgijevic Savvulidi, et al.
Published: (2021-02-01)

Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023
by: Filipp Georgijevič Savvulidi, et al.
Published: (2023-12-01)

Viabilitas Spermatozoa Ayam Hutan Hijau dalam Pengencer Posfat Kuning Telur Ditambah Laktosa pada Penyimpanan 5oC (GREEN JUNGGLE FOWL SPERM VIABILITY IN EGG YOLK PHOSFAT DILUENTS CONTAINING LACTOSE STORAGED AT 5OC)
by: Wayan Bebas, et al.
Published: (2015-05-01)

Effectiveness of Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo (Bubalus bubalis)
by: Yendraliza Yendraliza, et al.
Published: (2023-06-01)

PERBANDINGAN KUALITAS SPERMATOZOA SAPI JANTAN PASCA THAWING DENGAN PENGENCER YANG BERBEDA
by: Abdul Malik
Published: (2019-06-01)

Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing
by: Filipp Georgijevic Savvulidi, et al.
Published: (2023-12-01)

Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test
by: Sandro C. Esteves, et al.
Published: (2007-06-01)

New approaches for long-term conservation of rooster spermatozoa
by: Martina Janosikova, et al.
Published: (2023-02-01)

Monitoring of milk performance of Wallachian sheep grazed under traditional Carpathian management in Western Beskids location
by: Martin Ptáček, et al.
Published: (2023-12-01)

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility
by: Suherni Susilowati, et al.
Published: (2021-08-01)

Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C
by: Liuming Zhang, et al.
Published: (2023-09-01)

Effects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method
by: S.C. Esteves, et al.
Published: (2007-07-01)

Low-density lipoprotein supplementation improves the quality of Holstein bulls' insemination doses
by: Jan Pytlík, et al.
Published: (2023-02-01)

Melatonin supplementation improves the intactness of plasma membrane and acrosomal membrane of cryopreserved spermatozoa in Hariana bull
by: DILEEP KUMAR YADAV, et al.
Published: (2019-07-01)

The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue
by: Pei-Qi Zhang, et al.
Published: (2022-04-01)

Flow cytometry data on the effect of dimethylacetamide and N-methylacetamide used at different concentrations on the quality of cryopreserved chicken semen
by: Manuela Madeddu, et al.
Published: (2023-04-01)

Paternal Age Amplifies Cryopreservation-Induced Stress in Human Spermatozoa
by: Silvia Pérez Casasús, et al.
Published: (2024-04-01)

Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen
by: Jan Pytlík, et al.
Published: (2022-03-01)

Optimal time for laparoscopic intrauterine insemination performed on ewes detected in natural heat
by: Nurlan Malmakov, et al.
Published: (2022-10-01)

Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose
by: Kartini Eriani, et al.
Published: (2017-12-01)

Effect of sunflower lecithin on Kalahari Red goat semen during cryopreservation
by: Adeyanju Sakirat Opeyemi, et al.
Published: (2018-03-01)

Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken
by: Ai Ushiyama, et al.
Published: (2016-10-01)

Pilot scale development for purification of USP grade glycerol /
by: Ramlan Abd. Aziz, 1958-
Published: (2003)

Comparison of Commercial Poultry Semen Extenders Modified for Cryopreservation Procedure in the Genetic Resource Program of Czech Golden Spotted Hen
by: Kristýna Petričáková, et al.
Published: (2022-10-01)

Effect of Silymarin on Plasma Membrane and Acrosome of Sperm Treated with Aluminum Chloride
by: Hamid Reza Momeni, et al.
Published: (2015-07-01)

Artemisia vulgaris extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa
by: Sabina Bhandari, et al.
Published: (2021-01-01)

Effect of viable cryopreserved human amniotic membrane sutured to cornea on course of experimental bacterial keratitis
by: K.V. Sereda, et al.
Published: (2015-10-01)

Vital Analysis of Cryopreserved Sperm of Marbled Flounder, Pseudopleuronectes yokohamae
by: Shaharior Hossen, et al.
Published: (2021-06-01)

The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens
by: Yong Mao, et al.
Published: (2018-01-01)

Nitrosopumilus as main source of isoprenoid glycerol dialkyl glycerol tetraether lipids in the central Baltic Sea
by: Anna Katharina Wittenborn, et al.
Published: (2023-09-01)

Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol
by: Hsiu-Lien Herbie Lin, et al.
Published: (2024-03-01)

Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen
by: T. M. Kanimozhi, et al.
Published: (2023-03-01)

Core–Shell Microfiber Encapsulation Enables Glycerol-Free Cryopreservation of RBCs with High Hematocrit
by: Xianhui Qin, et al.
Published: (2023-11-01)

Cryopreservation in a Standard Freezer: −28 °C as Alternative Storage Temperature for Amniotic Membrane Transplantation
by: Joana Witt, et al.
Published: (2022-02-01)

Dynamics of mitochondrial membrane potential and DNA damage during cryopreservation of cattle and buffalo bull spermatozoa
by: KAMARAJ ELANGO, et al.
Published: (2021-07-01)

Pilot scale development for purification of USP grade glycerol /
by: Ramlan Abd. Aziz, 1958-, et al.
Published: (2006)

Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity
by: Z. Veznik, et al.
Published: (2003-08-01)

Functional sperm assessments of African Lion Panthera leo (Mammalia: Carnivora: Felidae) in field conditions
by: Thiesa Butterby Soler Barbosa, et al.
Published: (2019-01-01)

Damage assessment of the equine sperm membranes by fluorimetric technique
by: Eneiva Carla Carvalho Celeghini, et al.
Published: (2010-12-01)

Cryopreservation and freezability of epididymal and ejaculated stallion spermatozoa
by: TR TALLURI, et al.
Published: (2023-08-01)