Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

Summary: Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole s...

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Bibliographic Details
Main Authors: Keiko Sakamoto, Shubham Goel, Atsuko Funakoshi, Tetsuya Honda, Keisuke Nagao
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007589