There is no magic bullet: the importance of testing reference gene stability in RT-qPCR experiments across multiple closely related species

Reverse Transcriptase quantitative Polymerase Chain Reaction (RT-qPCR) is the current gold standard tool for the study of gene expression. This technique is highly dependent on the validation of reference genes, which exhibit stable expression levels among experimental conditions. Often, reference g...

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Bibliographic Details
Main Authors: Bert Foquet, Hojun Song
Format: Article
Language:English
Published: PeerJ Inc. 2020-08-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/9618.pdf