Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy.
Relief from painful diabetic neuropathy is an important clinical issue. We have previously shown that the transplantation of cultured endothelial progenitor cells or mesenchymal stem cells ameliorated diabetic neuropathy in rats. In this study, we investigated whether transplantation of freshly isol...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3220696?pdf=render |
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| author | Keiko Naruse Jun Sato Megumi Funakubo Masaki Hata Nobuhisa Nakamura Yasuko Kobayashi Hideki Kamiya Taiga Shibata Masaki Kondo Tatsuhito Himeno Tatsuaki Matsubara Yutaka Oiso Jiro Nakamura |
| author_facet | Keiko Naruse Jun Sato Megumi Funakubo Masaki Hata Nobuhisa Nakamura Yasuko Kobayashi Hideki Kamiya Taiga Shibata Masaki Kondo Tatsuhito Himeno Tatsuaki Matsubara Yutaka Oiso Jiro Nakamura |
| author_sort | Keiko Naruse |
| collection | DOAJ |
| description | Relief from painful diabetic neuropathy is an important clinical issue. We have previously shown that the transplantation of cultured endothelial progenitor cells or mesenchymal stem cells ameliorated diabetic neuropathy in rats. In this study, we investigated whether transplantation of freshly isolated bone marrow-derived mononuclear cells (BM-MNCs) alleviates neuropathic pain in the early stage of streptozotocin-induced diabetic rats. Two weeks after STZ injection, BM-MNCs or vehicle saline were injected into the unilateral hind limb muscles. Mechanical hyperalgesia and cold allodynia in SD rats were measured as the number of foot withdrawals to von Frey hair stimulation and acetone application, respectively. Two weeks after the BM-MNC transplantation, sciatic motor nerve conduction velocity (MNCV), sensory nerve conduction velocity (SNCV), sciatic nerve blood flow (SNBF), mRNA expressions and histology were assessed. The BM-MNC transplantation significantly ameliorated mechanical hyperalgesia and cold allodynia in the BM-MNC-injected side. Furthermore, the slowed MNCV/SNCV and decreased SNBF in diabetic rats were improved in the BM-MNC-injected side. BM-MNC transplantation improved the decreased mRNA expression of NT-3 and number of microvessels in the hind limb muscles. There was no distinct effect of BM-MNC transplantation on the intraepidermal nerve fiber density. These results suggest that autologous transplantation of BM-MNCs could be a novel strategy for the treatment of painful diabetic neuropathy. |
| first_indexed | 2024-12-21T16:47:37Z |
| format | Article |
| id | doaj.art-ec8b47f0d61240d9b797cd49414c4324 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T16:47:37Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-ec8b47f0d61240d9b797cd49414c43242022-12-21T18:56:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2745810.1371/journal.pone.0027458Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy.Keiko NaruseJun SatoMegumi FunakuboMasaki HataNobuhisa NakamuraYasuko KobayashiHideki KamiyaTaiga ShibataMasaki KondoTatsuhito HimenoTatsuaki MatsubaraYutaka OisoJiro NakamuraRelief from painful diabetic neuropathy is an important clinical issue. We have previously shown that the transplantation of cultured endothelial progenitor cells or mesenchymal stem cells ameliorated diabetic neuropathy in rats. In this study, we investigated whether transplantation of freshly isolated bone marrow-derived mononuclear cells (BM-MNCs) alleviates neuropathic pain in the early stage of streptozotocin-induced diabetic rats. Two weeks after STZ injection, BM-MNCs or vehicle saline were injected into the unilateral hind limb muscles. Mechanical hyperalgesia and cold allodynia in SD rats were measured as the number of foot withdrawals to von Frey hair stimulation and acetone application, respectively. Two weeks after the BM-MNC transplantation, sciatic motor nerve conduction velocity (MNCV), sensory nerve conduction velocity (SNCV), sciatic nerve blood flow (SNBF), mRNA expressions and histology were assessed. The BM-MNC transplantation significantly ameliorated mechanical hyperalgesia and cold allodynia in the BM-MNC-injected side. Furthermore, the slowed MNCV/SNCV and decreased SNBF in diabetic rats were improved in the BM-MNC-injected side. BM-MNC transplantation improved the decreased mRNA expression of NT-3 and number of microvessels in the hind limb muscles. There was no distinct effect of BM-MNC transplantation on the intraepidermal nerve fiber density. These results suggest that autologous transplantation of BM-MNCs could be a novel strategy for the treatment of painful diabetic neuropathy.http://europepmc.org/articles/PMC3220696?pdf=render |
| spellingShingle | Keiko Naruse Jun Sato Megumi Funakubo Masaki Hata Nobuhisa Nakamura Yasuko Kobayashi Hideki Kamiya Taiga Shibata Masaki Kondo Tatsuhito Himeno Tatsuaki Matsubara Yutaka Oiso Jiro Nakamura Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy. PLoS ONE |
| title | Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy. |
| title_full | Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy. |
| title_fullStr | Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy. |
| title_full_unstemmed | Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy. |
| title_short | Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy. |
| title_sort | transplantation of bone marrow derived mononuclear cells improves mechanical hyperalgesia cold allodynia and nerve function in diabetic neuropathy |
| url | http://europepmc.org/articles/PMC3220696?pdf=render |
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