The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human.
RNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA) in all eukaryotes, accounting for the major part of transcriptional activity in proliferating cells. Although basal Pol I transcription factors have been characterized in diverse organisms, the molecular basis of the robust rRNA production in...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-02-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC6415870?pdf=render |
| _version_ | 1818145753983352832 |
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| author | Katharina Hannig Virginia Babl Kristin Hergert Andreas Maier Michael Pilsl Christopher Schächner Ulrike Stöckl Philipp Milkereit Herbert Tschochner Wolfgang Seufert Joachim Griesenbeck |
| author_facet | Katharina Hannig Virginia Babl Kristin Hergert Andreas Maier Michael Pilsl Christopher Schächner Ulrike Stöckl Philipp Milkereit Herbert Tschochner Wolfgang Seufert Joachim Griesenbeck |
| author_sort | Katharina Hannig |
| collection | DOAJ |
| description | RNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA) in all eukaryotes, accounting for the major part of transcriptional activity in proliferating cells. Although basal Pol I transcription factors have been characterized in diverse organisms, the molecular basis of the robust rRNA production in vivo remains largely unknown. In S. cerevisiae, the multifunctional Net1 protein was reported to stimulate Pol I transcription. We found that the Pol I-stimulating function can be attributed to the very C-terminal region (CTR) of Net1. The CTR was required for normal cell growth and Pol I recruitment to rRNA genes in vivo and sufficient to promote Pol I transcription in vitro. Similarity with the acidic tail region of mammalian Pol I transcription factor UBF, which could partly functionally substitute for the CTR, suggests conserved roles for CTR-like domains in Pol I transcription from yeast to human. |
| first_indexed | 2024-12-11T12:08:29Z |
| format | Article |
| id | doaj.art-eccc7aa5c50a44fdb87e85e67cf887df |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-11T12:08:29Z |
| publishDate | 2019-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-eccc7aa5c50a44fdb87e85e67cf887df2022-12-22T01:07:52ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-02-01152e100800610.1371/journal.pgen.1008006The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human.Katharina HannigVirginia BablKristin HergertAndreas MaierMichael PilslChristopher SchächnerUlrike StöcklPhilipp MilkereitHerbert TschochnerWolfgang SeufertJoachim GriesenbeckRNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA) in all eukaryotes, accounting for the major part of transcriptional activity in proliferating cells. Although basal Pol I transcription factors have been characterized in diverse organisms, the molecular basis of the robust rRNA production in vivo remains largely unknown. In S. cerevisiae, the multifunctional Net1 protein was reported to stimulate Pol I transcription. We found that the Pol I-stimulating function can be attributed to the very C-terminal region (CTR) of Net1. The CTR was required for normal cell growth and Pol I recruitment to rRNA genes in vivo and sufficient to promote Pol I transcription in vitro. Similarity with the acidic tail region of mammalian Pol I transcription factor UBF, which could partly functionally substitute for the CTR, suggests conserved roles for CTR-like domains in Pol I transcription from yeast to human.http://europepmc.org/articles/PMC6415870?pdf=render |
| spellingShingle | Katharina Hannig Virginia Babl Kristin Hergert Andreas Maier Michael Pilsl Christopher Schächner Ulrike Stöckl Philipp Milkereit Herbert Tschochner Wolfgang Seufert Joachim Griesenbeck The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human. PLoS Genetics |
| title | The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human. |
| title_full | The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human. |
| title_fullStr | The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human. |
| title_full_unstemmed | The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human. |
| title_short | The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human. |
| title_sort | c terminal region of net1 is an activator of rna polymerase i transcription with conserved features from yeast to human |
| url | http://europepmc.org/articles/PMC6415870?pdf=render |
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