GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity
B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abundant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 18...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/3/494 |
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| author | Line Barington Liv von Voss Christensen Kristian Kåber Pedersen Kristine Niss Arfelt Martin Roumain Kristian Høj Reveles Jensen Viktoria Madeline Skovgaard Kjær Viktorija Daugvilaite John F. Kearney Jan Pravsgaard Christensen Gertrud Malene Hjortø Giulio G. Muccioli Peter Johannes Holst Mette Marie Rosenkilde |
| author_facet | Line Barington Liv von Voss Christensen Kristian Kåber Pedersen Kristine Niss Arfelt Martin Roumain Kristian Høj Reveles Jensen Viktoria Madeline Skovgaard Kjær Viktorija Daugvilaite John F. Kearney Jan Pravsgaard Christensen Gertrud Malene Hjortø Giulio G. Muccioli Peter Johannes Holst Mette Marie Rosenkilde |
| author_sort | Line Barington |
| collection | DOAJ |
| description | B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abundant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a- or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments. |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:03:47Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-ecdf797f65d7414b8242f2c44e167d782023-11-23T16:12:54ZengMDPI AGCells2073-44092022-01-0111349410.3390/cells11030494GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal CavityLine Barington0Liv von Voss Christensen1Kristian Kåber Pedersen2Kristine Niss Arfelt3Martin Roumain4Kristian Høj Reveles Jensen5Viktoria Madeline Skovgaard Kjær6Viktorija Daugvilaite7John F. Kearney8Jan Pravsgaard Christensen9Gertrud Malene Hjortø10Giulio G. Muccioli11Peter Johannes Holst12Mette Marie Rosenkilde13Laboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkBioanalysis and Pharmacology of Bioactive Lipids Research Group, Louvain Drug Research Institute, Université catholique de Louvain, 1200 Brussels, BelgiumLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkDivision of Developmental and Clinical Immunology, Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USAInfectious Immunology Group, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkBioanalysis and Pharmacology of Bioactive Lipids Research Group, Louvain Drug Research Institute, Université catholique de Louvain, 1200 Brussels, BelgiumExperimental Vaccinology Group, Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkLaboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkB1 cells constitute a specialized subset of B cells, best characterized in mice, which is abundant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a- or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.https://www.mdpi.com/2073-4409/11/3/494GPCR7TM receptorGPR183EBI2B1 cellB-1 cell |
| spellingShingle | Line Barington Liv von Voss Christensen Kristian Kåber Pedersen Kristine Niss Arfelt Martin Roumain Kristian Høj Reveles Jensen Viktoria Madeline Skovgaard Kjær Viktorija Daugvilaite John F. Kearney Jan Pravsgaard Christensen Gertrud Malene Hjortø Giulio G. Muccioli Peter Johannes Holst Mette Marie Rosenkilde GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity Cells GPCR 7TM receptor GPR183 EBI2 B1 cell B-1 cell |
| title | GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity |
| title_full | GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity |
| title_fullStr | GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity |
| title_full_unstemmed | GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity |
| title_short | GPR183 Is Dispensable for B1 Cell Accumulation and Function, but Affects B2 Cell Abundance, in the Omentum and Peritoneal Cavity |
| title_sort | gpr183 is dispensable for b1 cell accumulation and function but affects b2 cell abundance in the omentum and peritoneal cavity |
| topic | GPCR 7TM receptor GPR183 EBI2 B1 cell B-1 cell |
| url | https://www.mdpi.com/2073-4409/11/3/494 |
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