Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expression
Dysfunction of endothelial cells (EC) is important for atherosclerosis progression. The aim of this study was to evaluate the potential of ginger extract (GEx), 6-gingerol (6-G) and 6-shogaol (6-Sh) to reverse EC dysfunction and to investigate its mechanism of action, using cultured human EC incubat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-09-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618304274 |
| _version_ | 1818564182390341632 |
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| author | Laura Toma Mina Raileanu Mariana Deleanu Camelia S. Stancu Anca V. Sima |
| author_facet | Laura Toma Mina Raileanu Mariana Deleanu Camelia S. Stancu Anca V. Sima |
| author_sort | Laura Toma |
| collection | DOAJ |
| description | Dysfunction of endothelial cells (EC) is important for atherosclerosis progression. The aim of this study was to evaluate the potential of ginger extract (GEx), 6-gingerol (6-G) and 6-shogaol (6-Sh) to reverse EC dysfunction and to investigate its mechanism of action, using cultured human EC incubated with TNFα. The results showed that GEx decreases monocyte chemoattractant protein-1, vascular cell adhesion molecule-1 and monocyte adhesion. This decrease was associated with the: (1) decrease of ninjurin-1 expression; (2) reduction of TNFα receptor1 and of the receptor for advanced glycation end-products expression (RAGE), in parallel with the increase of soluble RAGE; (3) decrease of NADPH oxidase subunits expression; (4) activation of antioxidant Nrf2 and heme oxygenase-1, and (5) inhibition of NF-kB. The benefic effects of 6-G and 6-Sh were weaker than those of GEx (GEx > 6-Sh > 6-G). In conclusion, GEx might be a promising alternative to ameliorate disorders in which oxidative stress and inflammation are important. |
| first_indexed | 2024-12-14T01:25:49Z |
| format | Article |
| id | doaj.art-ece9eb436f094b5397f8fd2f9b9829db |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T01:25:49Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ece9eb436f094b5397f8fd2f9b9829db2022-12-21T23:22:12ZengElsevierJournal of Functional Foods1756-46462018-09-0148654664Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expressionLaura Toma0Mina Raileanu1Mariana Deleanu2Camelia S. Stancu3Anca V. Sima4Lipidomics Department, Institute of Cellular Biology and Pathology “Nicolae Simionescu” of the Romanian Academy, Bucharest, RomaniaLipidomics Department, Institute of Cellular Biology and Pathology “Nicolae Simionescu” of the Romanian Academy, Bucharest, RomaniaLipidomics Department, Institute of Cellular Biology and Pathology “Nicolae Simionescu” of the Romanian Academy, Bucharest, Romania; Faculty of Biotechnology, University of Agronomical Sciences and Veterinary Medicine, Bucharest, RomaniaLipidomics Department, Institute of Cellular Biology and Pathology “Nicolae Simionescu” of the Romanian Academy, Bucharest, RomaniaLipidomics Department, Institute of Cellular Biology and Pathology “Nicolae Simionescu” of the Romanian Academy, Bucharest, Romania; Corresponding author at: Lipidomics Department, Institute of Cellular Biology and Pathology “Nicolae Simionescu” of the Romanian Academy, 8,B.P. Hasdeu Street, 050568 Bucharest, Romania.Dysfunction of endothelial cells (EC) is important for atherosclerosis progression. The aim of this study was to evaluate the potential of ginger extract (GEx), 6-gingerol (6-G) and 6-shogaol (6-Sh) to reverse EC dysfunction and to investigate its mechanism of action, using cultured human EC incubated with TNFα. The results showed that GEx decreases monocyte chemoattractant protein-1, vascular cell adhesion molecule-1 and monocyte adhesion. This decrease was associated with the: (1) decrease of ninjurin-1 expression; (2) reduction of TNFα receptor1 and of the receptor for advanced glycation end-products expression (RAGE), in parallel with the increase of soluble RAGE; (3) decrease of NADPH oxidase subunits expression; (4) activation of antioxidant Nrf2 and heme oxygenase-1, and (5) inhibition of NF-kB. The benefic effects of 6-G and 6-Sh were weaker than those of GEx (GEx > 6-Sh > 6-G). In conclusion, GEx might be a promising alternative to ameliorate disorders in which oxidative stress and inflammation are important.http://www.sciencedirect.com/science/article/pii/S1756464618304274Endothelial dysfunctionGinger extractNinjurin-1TNFR1NADPH oxidaseRAGE |
| spellingShingle | Laura Toma Mina Raileanu Mariana Deleanu Camelia S. Stancu Anca V. Sima Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expression Journal of Functional Foods Endothelial dysfunction Ginger extract Ninjurin-1 TNFR1 NADPH oxidase RAGE |
| title | Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expression |
| title_full | Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expression |
| title_fullStr | Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expression |
| title_full_unstemmed | Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expression |
| title_short | Novel molecular mechanisms by which ginger extract reduces the inflammatory stress in TNFα – activated human endothelial cells; decrease of Ninjurin-1, TNFR1 and NADPH oxidase subunits expression |
| title_sort | novel molecular mechanisms by which ginger extract reduces the inflammatory stress in tnfα activated human endothelial cells decrease of ninjurin 1 tnfr1 and nadph oxidase subunits expression |
| topic | Endothelial dysfunction Ginger extract Ninjurin-1 TNFR1 NADPH oxidase RAGE |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618304274 |
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