Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel
Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vit...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/24/2/320 |
| _version_ | 1818277940261027840 |
|---|---|
| author | Nur Fatimah Lasano Azizah Haji Hamid Roselina Karim Mohd Sabri Pak Dek Radhiah Shukri Nurul Shazini Ramli |
| author_facet | Nur Fatimah Lasano Azizah Haji Hamid Roselina Karim Mohd Sabri Pak Dek Radhiah Shukri Nurul Shazini Ramli |
| author_sort | Nur Fatimah Lasano |
| collection | DOAJ |
| description | Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vitamins and minerals analyses), the anti-diabetic activities and phytochemical profile of M. odorata peel and seed kernel were investigated for the first time. The results indicated that seed kernel rich in fat, protein, carbohydrate, and ash while peel contained significantly greater amount of fiber, minerals, β-Carotene and ascorbic acid compared to seed kernel. The samples were then extracted using different solvents (acetone, ethanol, methanol at 60%, v/v and pure deionized water) and their anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Seed kernel had the lowest IC50 values for α-amylase and α-glucosidase inhibition assay in 60% ethanol and 60% acetone, respectively. Due to the toxic effect and high volatility of acetone, the ethanolic extracts of samples were further analyses for their phytochemical profile using high performance liquid chromatography-mass spectrometry (LC-MS) and ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). The most abundant compounds identified were phenolic acid, ellagic acid, and flavonoid. These findings suggest that M. odorata fruit wastes, especially the seed kernel possesses promising ability to be used as functional ingredient in the food industry. |
| first_indexed | 2024-12-12T23:09:31Z |
| format | Article |
| id | doaj.art-ecf3071b79504a74b16a20283839bbca |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T23:09:31Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ecf3071b79504a74b16a20283839bbca2022-12-22T00:08:37ZengMDPI AGMolecules1420-30492019-01-0124232010.3390/molecules24020320molecules24020320Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed KernelNur Fatimah Lasano0Azizah Haji Hamid1Roselina Karim2Mohd Sabri Pak Dek3Radhiah Shukri4Nurul Shazini Ramli5Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaDepartment of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaDepartment of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaMangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vitamins and minerals analyses), the anti-diabetic activities and phytochemical profile of M. odorata peel and seed kernel were investigated for the first time. The results indicated that seed kernel rich in fat, protein, carbohydrate, and ash while peel contained significantly greater amount of fiber, minerals, β-Carotene and ascorbic acid compared to seed kernel. The samples were then extracted using different solvents (acetone, ethanol, methanol at 60%, v/v and pure deionized water) and their anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Seed kernel had the lowest IC50 values for α-amylase and α-glucosidase inhibition assay in 60% ethanol and 60% acetone, respectively. Due to the toxic effect and high volatility of acetone, the ethanolic extracts of samples were further analyses for their phytochemical profile using high performance liquid chromatography-mass spectrometry (LC-MS) and ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). The most abundant compounds identified were phenolic acid, ellagic acid, and flavonoid. These findings suggest that M. odorata fruit wastes, especially the seed kernel possesses promising ability to be used as functional ingredient in the food industry.http://www.mdpi.com/1420-3049/24/2/320Kuinifruitwastesvitaminsmineralsphenolicsα-amylaseα-glucosidase |
| spellingShingle | Nur Fatimah Lasano Azizah Haji Hamid Roselina Karim Mohd Sabri Pak Dek Radhiah Shukri Nurul Shazini Ramli Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel Molecules Kuini fruit wastes vitamins minerals phenolics α-amylase α-glucosidase |
| title | Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel |
| title_full | Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel |
| title_fullStr | Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel |
| title_full_unstemmed | Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel |
| title_short | Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel |
| title_sort | nutritional composition anti diabetic properties and identification of active compounds using uhplc esi orbitrap ms ms in mangifera odorata l peel and seed kernel |
| topic | Kuini fruit wastes vitamins minerals phenolics α-amylase α-glucosidase |
| url | http://www.mdpi.com/1420-3049/24/2/320 |
| work_keys_str_mv | AT nurfatimahlasano nutritionalcompositionantidiabeticpropertiesandidentificationofactivecompoundsusinguhplcesiorbitrapmsmsinmangiferaodoratalpeelandseedkernel AT azizahhajihamid nutritionalcompositionantidiabeticpropertiesandidentificationofactivecompoundsusinguhplcesiorbitrapmsmsinmangiferaodoratalpeelandseedkernel AT roselinakarim nutritionalcompositionantidiabeticpropertiesandidentificationofactivecompoundsusinguhplcesiorbitrapmsmsinmangiferaodoratalpeelandseedkernel AT mohdsabripakdek nutritionalcompositionantidiabeticpropertiesandidentificationofactivecompoundsusinguhplcesiorbitrapmsmsinmangiferaodoratalpeelandseedkernel AT radhiahshukri nutritionalcompositionantidiabeticpropertiesandidentificationofactivecompoundsusinguhplcesiorbitrapmsmsinmangiferaodoratalpeelandseedkernel AT nurulshaziniramli nutritionalcompositionantidiabeticpropertiesandidentificationofactivecompoundsusinguhplcesiorbitrapmsmsinmangiferaodoratalpeelandseedkernel |