CRISPRi is not strand-specific at all loci and redefines the transcriptional landscape

CRISPRi is not strand-specific at all loci and redefines the transcriptional landscape

CRISPRi, an adapted CRISPR-Cas9 system, is proposed to act as a strand-specific roadblock to repress transcription in eukaryotic cells using guide RNAs (sgRNAs) to target catalytically inactive Cas9 (dCas9) and offers an alternative to genetic interventions for studying pervasive antisense transcrip...

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Bibliographic Details
Main Authors: Françoise S Howe, Andrew Russell, Anna R Lamstaes, Afaf El-Sagheer, Anitha Nair, Tom Brown, Jane Mellor
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-10-01
Series:eLife
Subjects:
CRISPRi
transcription roadblock
strand-specificity
transcription initiation
transcription termination
HMS2 and GAL1
Online Access:https://elifesciences.org/articles/29878

Internet

https://elifesciences.org/articles/29878

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