Gene-centric coverage of the human liver transcriptome: QPCR, Illumina, and Oxford Nanopore RNA-Seq

Gene-centric coverage of the human liver transcriptome: QPCR, Illumina, and Oxford Nanopore RNA-Seq

It has been shown that the best coverage of the HepG2 cell line transcriptome encoded by genes of a single chromosome, chromosome 18, is achieved by a combination of two sequencing platforms, Illumina RNA-Seq and Oxford Nanopore Technologies (ONT), using cut-off levels of FPKM > 0 and TPM &am...

Full description

Bibliographic Details
Main Authors: Ekaterina V. Ilgisonis, Elena A. Ponomarenko, Svetlana N. Tarbeeva, Andrey V. Lisitsa, Victor G. Zgoda, Sergey P. Radko, Alexander I. Archakov
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-12-01
Series:Frontiers in Molecular Biosciences
Subjects:
transcriptomics
human genome
qPCR
Illumina RNA-Seq
Oxford Nanopore Technologies MinION
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.944639/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2022.944639/full

Similar Items