Sortase-mediated immobilization of Candida antarctica lipase B (CalB) on graphene oxide; comparison with chemical approach

In this study, Candida antarctica lipase B (CalB) was covalently immobilized on the surface of graphene oxide (GO) nanoparticles by sortase-mediated immobilization as well as a chemical attachment approach. Sortase is a transpeptidase that provides one-step purification and targeted immobilization o...

Full description

Bibliographic Details
Main Authors: Faezeh Moosavi, Faezeh Ahrari, Gholamreza Ahmadian, Mehdi Mohammadi
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:Biotechnology Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X22000327