A Novel Strategy of US3 Codon De-Optimization for Construction of an Attenuated Pseudorabies Virus against High Virulent Chinese Pseudorabies Virus Variant

A Novel Strategy of US3 Codon De-Optimization for Construction of an Attenuated Pseudorabies Virus against High Virulent Chinese Pseudorabies Virus Variant

In this study, we applied bacterial artificial chromosome (BAC) technology with PRV<sup>ΔTK/gE/gI</sup> as the base material to replace the first, central, and terminal segments of the US3 gene with codon-deoptimized fragments via two-step Red-mediated recombination in <i>E. coli&l...

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Bibliographic Details
Main Authors: Mengwei Xu, Yiwei Wang, Yamei Liu, Saisai Chen, Laixu Zhu, Ling Tong, Yating Zheng, Nikolaus Osterrieder, Chuanjian Zhang, Jichun Wang
Format: Article
Language:English
Published: MDPI AG 2023-07-01
Series:Vaccines
Subjects:
pseudorabies virus
virulence
safety
immunogenicity
codon de-optimization
Online Access:https://www.mdpi.com/2076-393X/11/8/1288

Internet

https://www.mdpi.com/2076-393X/11/8/1288

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