Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samples
Phenolic compounds occur in effluents from a wide variety of industrial operations, and they present toxicity and generate environmental problems. The treatment of these wastes via enzyme immobilization indicates that peroxidase (E.C.1.11.1.7) is the enzyme with the widest application. In the same p...
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| Format: | Article |
| Language: | English |
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Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi)
2018-11-01
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| Series: | Revista Ambiente & Água |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2018000600306&lng=pt&nrm=iso&tlng=en |
| _version_ | 1819211510205906944 |
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| author | Thâmara Machado e Silva Aline Rodrigues dos Santos Samantha Salomão Caramori |
| author_facet | Thâmara Machado e Silva Aline Rodrigues dos Santos Samantha Salomão Caramori |
| author_sort | Thâmara Machado e Silva |
| collection | DOAJ |
| description | Phenolic compounds occur in effluents from a wide variety of industrial operations, and they present toxicity and generate environmental problems. The treatment of these wastes via enzyme immobilization indicates that peroxidase (E.C.1.11.1.7) is the enzyme with the widest application. In the same process, biodegradable polymers have generated significant interest from research and industrial communities. Here we applied biopolymers from sugarcane bagasse (SB) and Cerrado cashew-tree polysaccharide (PEJU-GO) as supports for peroxidase immobilization from crude green zucchini extract via physical adsorption and covalent bonding. In addition, we used these systems in the removal of phenols from industrial effluents. Electron microscopy showed a fibrous surface for SB and a microporous material for PEJU-GO, characteristics that facilitate the immobilization. The infrared of both materials elucidated characteristic bands of sugars. For operational stability after 30 days of storage, the immobilized peroxidase retained 69 and 50% activity for SB and PEJU-GO, respectively. The systems were able to remove up to 79% of phenolics from industrial wastes. Therefore, the biopolymers from sugarcane bagasse and cashew-tree polysaccharide are promising sources for biotechnology, especially when used for peroxidase immobilization. This will support enzymatic activity in laboratory practice, with homogeneous behavior in different pH conditions, at low cost, biodegradable, non-toxic and extracted in a sustainable way. |
| first_indexed | 2024-12-23T06:28:13Z |
| format | Article |
| id | doaj.art-edd547eb883b474e9a1e513738ae1e54 |
| institution | Directory Open Access Journal |
| issn | 1980-993X |
| language | English |
| last_indexed | 2024-12-23T06:28:13Z |
| publishDate | 2018-11-01 |
| publisher | Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi) |
| record_format | Article |
| series | Revista Ambiente & Água |
| spelling | doaj.art-edd547eb883b474e9a1e513738ae1e542022-12-21T17:57:00ZengInstituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi)Revista Ambiente & Água1980-993X2018-11-0113611210.4136/ambi-agua.2226Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samplesThâmara Machado e Silva0Aline Rodrigues dos Santos1Samantha Salomão Caramori2Universidade Estadual de Goiás (UEG), Anápolis, GO, Brasil Câmpus Anápolis de Ciências Exatas e Tecnológicas (CCET). Laboratório de BiotecnologiaUniversidade Estadual de Goiás (UEG), Anápolis, GO, Brasil Câmpus Anápolis de Ciências Exatas e Tecnológicas (CCET). Laboratório de BiotecnologiaUniversidade Estadual de Goiás (UEG), Anápolis, GO, Brasil Câmpus Anápolis de Ciências Exatas e Tecnológicas (CCET). Laboratório de BiotecnologiaPhenolic compounds occur in effluents from a wide variety of industrial operations, and they present toxicity and generate environmental problems. The treatment of these wastes via enzyme immobilization indicates that peroxidase (E.C.1.11.1.7) is the enzyme with the widest application. In the same process, biodegradable polymers have generated significant interest from research and industrial communities. Here we applied biopolymers from sugarcane bagasse (SB) and Cerrado cashew-tree polysaccharide (PEJU-GO) as supports for peroxidase immobilization from crude green zucchini extract via physical adsorption and covalent bonding. In addition, we used these systems in the removal of phenols from industrial effluents. Electron microscopy showed a fibrous surface for SB and a microporous material for PEJU-GO, characteristics that facilitate the immobilization. The infrared of both materials elucidated characteristic bands of sugars. For operational stability after 30 days of storage, the immobilized peroxidase retained 69 and 50% activity for SB and PEJU-GO, respectively. The systems were able to remove up to 79% of phenolics from industrial wastes. Therefore, the biopolymers from sugarcane bagasse and cashew-tree polysaccharide are promising sources for biotechnology, especially when used for peroxidase immobilization. This will support enzymatic activity in laboratory practice, with homogeneous behavior in different pH conditions, at low cost, biodegradable, non-toxic and extracted in a sustainable way.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2018000600306&lng=pt&nrm=iso&tlng=enbiopolymerseffluentswater treatment |
| spellingShingle | Thâmara Machado e Silva Aline Rodrigues dos Santos Samantha Salomão Caramori Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samples Revista Ambiente & Água biopolymers effluents water treatment |
| title | Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samples |
| title_full | Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samples |
| title_fullStr | Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samples |
| title_full_unstemmed | Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samples |
| title_short | Peroxidase from green zucchini (Cucurbita pepo L.) immobilized on natural polymers removes phenolic compounds from water samples |
| title_sort | peroxidase from green zucchini cucurbita pepo l immobilized on natural polymers removes phenolic compounds from water samples |
| topic | biopolymers effluents water treatment |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2018000600306&lng=pt&nrm=iso&tlng=en |
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