A simple and rapid technique of template preparation for PCR

A simple and rapid technique of template preparation for PCR

Many techniques have been developed for extracting DNA, but most are often complex, time-consuming, and/or expensive. In this study, we describe a simple, rapid and cost-effective technique for preparing DNA template for PCR. This technique involves 0.1 M potassium hydroxide treatment at 100°C for 1...

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Bibliographic Details
Main Authors: Yunyun Liu, Jia Chen, Yi Cheng, Yi Li, Xinwen Li, Zhengbing Zhang, Xiumei Xu, Yufeng Lin, Jianping Xu, Zhimin Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Microbiology
Subjects:
simple PCR template preparation
microorganism
potassium hydroxide
long fragment amplification
high through-put PCR
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.1024827/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.1024827/full

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