<i>Agrobacterium tumefaciens</i>-Mediated Transformation of NHEJ Mutant <i>Aspergillus nidulans</i> Conidia: An Efficient Tool for Targeted Gene Recombination Using Selectable Nutritional Markers

<i>Agrobacterium tumefaciens</i>-Mediated Transformation of NHEJ Mutant <i>Aspergillus nidulans</i> Conidia: An Efficient Tool for Targeted Gene Recombination Using Selectable Nutritional Markers

Protoplast transformation for the introduction of recombinant DNA into <i>Aspergillus nidulans</i> is technically demanding and dependant on the availability and batch variability of commercial enzyme preparations. Given the success of <i>Agrobacterium tumefaciens</i>-mediate...

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Bibliographic Details
Main Authors: Virginia Casado-del Castillo, Andrew P. MacCabe, Margarita Orejas
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:Journal of Fungi
Subjects:
<i>Aspergillus nidulans</i>
conidia
fungal transformation
ATMT
pCAMBIA-derived vectors
targeted gene deletion/disruption
Online Access:https://www.mdpi.com/2309-608X/7/11/961

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https://www.mdpi.com/2309-608X/7/11/961

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