Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages
Influenza A virus infection (IAV) often leads to acute lung injury that impairs breathing and can lead to death, with disproportionate mortality in children and the elderly. Surfactant Protein A (SP-A) is a calcium-dependent opsonin that binds a variety of pathogens to help control pulmonary infect...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.919800/full |
| _version_ | 1811158947068903424 |
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| author | Eric Yau Linlin Yang Yan Chen Todd M. Umstead Hannah Atkins Zoe E. Katz Jonathan W. Yewdell Chintan K. Gandhi E. Scott Halstead Zissis C. Chroneos Zissis C. Chroneos |
| author_facet | Eric Yau Linlin Yang Yan Chen Todd M. Umstead Hannah Atkins Zoe E. Katz Jonathan W. Yewdell Chintan K. Gandhi E. Scott Halstead Zissis C. Chroneos Zissis C. Chroneos |
| author_sort | Eric Yau |
| collection | DOAJ |
| description | Influenza A virus infection (IAV) often leads to acute lung injury that impairs breathing and can lead to death, with disproportionate mortality in children and the elderly. Surfactant Protein A (SP-A) is a calcium-dependent opsonin that binds a variety of pathogens to help control pulmonary infections by alveolar macrophages. Alveolar macrophages play critical roles in host resistance and susceptibility to IAV infection. The effect of SP-A on IAV infection and antiviral response of macrophages, however, is not understood. Here, we report that SP-A attenuates IAV infection in a dose-dependent manner at the level of endosomal trafficking, resulting in infection delay in a model macrophage cell line. The ability of SP-A to suppress infection was independent of its glycosylation status. Binding of SP-A to hemagglutinin did not rely on the glycosylation status or sugar binding properties of either protein. Incubation of either macrophages or IAV with SP-A slowed endocytic uptake rate of IAV. SP-A interfered with binding to cell membrane and endosomal exit of the viral genome as indicated by experiments using isolated cell membranes, an antibody recognizing a pH-sensitive conformational epitope on hemagglutinin, and microscopy. Lack of SP-A in mice enhanced IFNβ expression, viral clearance and reduced mortality from IAV infection. These findings support the idea that IAV is an opportunistic pathogen that co-opts SP-A to evade host defense by alveolar macrophages. Our study highlights novel aspects of host-pathogen interactions that may lead to better understanding of the local mechanisms that shape activation of antiviral and inflammatory responses to viral infection in the lung. |
| first_indexed | 2024-04-10T05:32:56Z |
| format | Article |
| id | doaj.art-ee1c29f7544b428881aed0e21bc94850 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T05:32:56Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-ee1c29f7544b428881aed0e21bc948502023-03-07T06:00:25ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-03-011410.3389/fimmu.2023.919800919800Surfactant protein A alters endosomal trafficking of influenza A virus in macrophagesEric Yau0Linlin Yang1Yan Chen2Todd M. Umstead3Hannah Atkins4Zoe E. Katz5Jonathan W. Yewdell6Chintan K. Gandhi7E. Scott Halstead8Zissis C. Chroneos9Zissis C. Chroneos10Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Comparative Medicine, Pennsylvania State University College of Medicine, PA, Hershey, United StatesDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesCellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD, United StatesDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Pediatrics, Division of Pediatric Critical Care Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, Pulmonary Immunology and Physiology Laboratory, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, PA, United StatesInfluenza A virus infection (IAV) often leads to acute lung injury that impairs breathing and can lead to death, with disproportionate mortality in children and the elderly. Surfactant Protein A (SP-A) is a calcium-dependent opsonin that binds a variety of pathogens to help control pulmonary infections by alveolar macrophages. Alveolar macrophages play critical roles in host resistance and susceptibility to IAV infection. The effect of SP-A on IAV infection and antiviral response of macrophages, however, is not understood. Here, we report that SP-A attenuates IAV infection in a dose-dependent manner at the level of endosomal trafficking, resulting in infection delay in a model macrophage cell line. The ability of SP-A to suppress infection was independent of its glycosylation status. Binding of SP-A to hemagglutinin did not rely on the glycosylation status or sugar binding properties of either protein. Incubation of either macrophages or IAV with SP-A slowed endocytic uptake rate of IAV. SP-A interfered with binding to cell membrane and endosomal exit of the viral genome as indicated by experiments using isolated cell membranes, an antibody recognizing a pH-sensitive conformational epitope on hemagglutinin, and microscopy. Lack of SP-A in mice enhanced IFNβ expression, viral clearance and reduced mortality from IAV infection. These findings support the idea that IAV is an opportunistic pathogen that co-opts SP-A to evade host defense by alveolar macrophages. Our study highlights novel aspects of host-pathogen interactions that may lead to better understanding of the local mechanisms that shape activation of antiviral and inflammatory responses to viral infection in the lung.https://www.frontiersin.org/articles/10.3389/fimmu.2023.919800/fullsurfactant protein Amacrophagesinfluenza A viruslungcollectin |
| spellingShingle | Eric Yau Linlin Yang Yan Chen Todd M. Umstead Hannah Atkins Zoe E. Katz Jonathan W. Yewdell Chintan K. Gandhi E. Scott Halstead Zissis C. Chroneos Zissis C. Chroneos Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages Frontiers in Immunology surfactant protein A macrophages influenza A virus lung collectin |
| title | Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages |
| title_full | Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages |
| title_fullStr | Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages |
| title_full_unstemmed | Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages |
| title_short | Surfactant protein A alters endosomal trafficking of influenza A virus in macrophages |
| title_sort | surfactant protein a alters endosomal trafficking of influenza a virus in macrophages |
| topic | surfactant protein A macrophages influenza A virus lung collectin |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.919800/full |
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