Selection of Ideal Reference Genes for Gene Expression Analysis in COVID-19 and Mucormycosis

Selection of Ideal Reference Genes for Gene Expression Analysis in COVID-19 and Mucormycosis

ABSTRACT Selection of reference genes during real-time quantitative PCR (qRT-PCR) is critical to determine accurate and reliable mRNA expression. Nonetheless, not a single study has investigated the expression stability of candidate reference genes to determine their suitability as internal controls...

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Bibliographic Details
Main Authors: Sunil Kumar, Ayaan Ahmad, Namrata Kushwaha, Niti Shokeen, Sheetal Negi, Kamini Gautam, Anup Singh, Pavan Tiwari, Rakesh Garg, Richa Agarwal, Anant Mohan, Anjan Trikha, Alok Thakar, Vikram Saini
Format: Article
Language:English
Published: American Society for Microbiology 2022-12-01
Series:Microbiology Spectrum
Subjects:
gene expression
qRT-PCR
reference gene
SARS-CoV-2
COVID-19
mucormycosis
Online Access:https://journals.asm.org/doi/10.1128/spectrum.01656-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.01656-22

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