Tailored Sample Mounting for Light-Sheet Fluorescence Microscopy of Clarified Specimens by Polydimethylsiloxane Casting

Tailored Sample Mounting for Light-Sheet Fluorescence Microscopy of Clarified Specimens by Polydimethylsiloxane Casting

The combination of biological tissue clearing methods with light-sheet fluorescence microscopy (LSFM) allows acquiring images of specific biological structures of interest at whole organ scale and microscopic resolution. Differently to classical epifluorescence techniques, where the sample is cut in...

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Bibliographic Details
Main Authors: Antonino Paolo Di Giovanna, Caterina Credi, Alessandra Franceschini, Marie Caroline Müllenbroich, Ludovico Silvestri, Francesco Saverio Pavone
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Neuroanatomy
Subjects:
light-sheet microscopy
tissue clearing
brain
sample mounting
PDMS
Online Access:https://www.frontiersin.org/article/10.3389/fnana.2019.00035/full

Internet

https://www.frontiersin.org/article/10.3389/fnana.2019.00035/full

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