Statistical differences resulting from selection of stable reference genes after hypoxia and hypothermia in the neonatal rat brain.

Real-time reverse transcription PCR (qPCR) normalized to an internal reference gene (RG), is a frequently used method for quantifying gene expression changes in neuroscience. Although RG expression is assumed to be constant independent of physiological or experimental conditions, several studies hav...

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Bibliographic Details
Main Authors: Martín Bustelo, Martín A Bruno, César F Loidl, Manuel Rey-Funes, Harry W M Steinbusch, Antonio W D Gavilanes, D L A van den Hove
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0233387