Three methods for examining the de novo proteome of microglia using BONCAT bioorthogonal labeling and FUNCAT click chemistry

Three methods for examining the de novo proteome of microglia using BONCAT bioorthogonal labeling and FUNCAT click chemistry

Summary: Bioorthogonal labeling and click chemistry techniques allow the detailed examination of cellular physiology through tagging and visualizing newly synthesized proteins. Here, we describe three methods applying bioorthogonal non-canonical amino acid tagging and fluorescent non-canonical amino...

Full description

Bibliographic Details
Main Authors: Alison Keolani Carlisle, Jürgen Götz, Liviu-Gabriel Bodea
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell Culture
Flow Cytometry/Mass Cytometry
Cell-based Assays
Microscopy
Neuroscience
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003854

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723003854

Similar Items