Safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig model
Abstract Background Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are often associated with airway fluid acidification. Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene leads to impaired bicarbonate secretion contributing to CF airway pathology...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Pulmonary Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12890-022-01919-x |
| _version_ | 1819022627436494848 |
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| author | Kata Csekő Dóra Hargitai Lilla Draskóczi Adrienn Kéri Pongsiri Jaikumpun Beáta Kerémi Zsuzsanna Helyes Ákos Zsembery |
| author_facet | Kata Csekő Dóra Hargitai Lilla Draskóczi Adrienn Kéri Pongsiri Jaikumpun Beáta Kerémi Zsuzsanna Helyes Ákos Zsembery |
| author_sort | Kata Csekő |
| collection | DOAJ |
| description | Abstract Background Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are often associated with airway fluid acidification. Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene leads to impaired bicarbonate secretion contributing to CF airway pathology. Chronic cigarette smoke (CS) -the major cause of COPD- is reported to induce acquired CFTR dysfunction underlying airway acidification and inflammation. We hypothesize that bicarbonate-containing aerosols could be beneficial for patients with CFTR dysfunctions. Thus, we investigated the safety of hypertonic sodium bicarbonate (NaHCO3) inhalation in CS-exposed guinea pigs. Methods Animals were divided into groups inhaling hypertonic NaCl (8.4%) or hypertonic NaHCO3 (8.4%) aerosol for 8 weeks. Subgroups from each treatment groups were further exposed to CS. Respiratory functions were measured at 0 and after 2, 4, 6 and 8 weeks. After 8 weeks blood tests and pulmonary histopathological assessment were performed. Results Neither smoking nor NaHCO3-inhalation affected body weight, arterial and urine pH, or histopathology significantly. NaHCO3-inhalation did not worsen respiratory parameters. Moreover, it normalized the CS-induced transient alterations in frequency, peak inspiratory flow, inspiratory and expiratory times. Conclusion Long-term NaHCO3-inhalation is safe in chronic CS-exposed guinea pigs. Our data suggest that bicarbonate-containing aerosols might be carefully applied to CF patients. |
| first_indexed | 2024-12-21T04:26:00Z |
| format | Article |
| id | doaj.art-ee79cdecf15e46c3a99f5f1fbc45ab6c |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-12-21T04:26:00Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-ee79cdecf15e46c3a99f5f1fbc45ab6c2022-12-21T19:16:03ZengBMCBMC Pulmonary Medicine1471-24662022-04-0122111010.1186/s12890-022-01919-xSafety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig modelKata Csekő0Dóra Hargitai1Lilla Draskóczi2Adrienn Kéri3Pongsiri Jaikumpun4Beáta Kerémi5Zsuzsanna Helyes6Ákos Zsembery7Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs2nd Department of Pathology, Semmelweis UniversityDepartment of Pharmacology and Pharmacotherapy, Medical School, University of PécsDepartment of Oral Biology, Faculty of Dentistry, Semmelweis UniversityDepartment of Oral Biology, Faculty of Dentistry, Semmelweis UniversityDepartment of Oral Biology, Faculty of Dentistry, Semmelweis UniversityDepartment of Pharmacology and Pharmacotherapy, Medical School, University of PécsDepartment of Oral Biology, Faculty of Dentistry, Semmelweis UniversityAbstract Background Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are often associated with airway fluid acidification. Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene leads to impaired bicarbonate secretion contributing to CF airway pathology. Chronic cigarette smoke (CS) -the major cause of COPD- is reported to induce acquired CFTR dysfunction underlying airway acidification and inflammation. We hypothesize that bicarbonate-containing aerosols could be beneficial for patients with CFTR dysfunctions. Thus, we investigated the safety of hypertonic sodium bicarbonate (NaHCO3) inhalation in CS-exposed guinea pigs. Methods Animals were divided into groups inhaling hypertonic NaCl (8.4%) or hypertonic NaHCO3 (8.4%) aerosol for 8 weeks. Subgroups from each treatment groups were further exposed to CS. Respiratory functions were measured at 0 and after 2, 4, 6 and 8 weeks. After 8 weeks blood tests and pulmonary histopathological assessment were performed. Results Neither smoking nor NaHCO3-inhalation affected body weight, arterial and urine pH, or histopathology significantly. NaHCO3-inhalation did not worsen respiratory parameters. Moreover, it normalized the CS-induced transient alterations in frequency, peak inspiratory flow, inspiratory and expiratory times. Conclusion Long-term NaHCO3-inhalation is safe in chronic CS-exposed guinea pigs. Our data suggest that bicarbonate-containing aerosols might be carefully applied to CF patients.https://doi.org/10.1186/s12890-022-01919-xAirwaysCystic fibrosisCOPDBicarbonatepHInhalation |
| spellingShingle | Kata Csekő Dóra Hargitai Lilla Draskóczi Adrienn Kéri Pongsiri Jaikumpun Beáta Kerémi Zsuzsanna Helyes Ákos Zsembery Safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig model BMC Pulmonary Medicine Airways Cystic fibrosis COPD Bicarbonate pH Inhalation |
| title | Safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig model |
| title_full | Safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig model |
| title_fullStr | Safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig model |
| title_full_unstemmed | Safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig model |
| title_short | Safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke-induced airway irritation guinea pig model |
| title_sort | safety of chronic hypertonic bicarbonate inhalation in a cigarette smoke induced airway irritation guinea pig model |
| topic | Airways Cystic fibrosis COPD Bicarbonate pH Inhalation |
| url | https://doi.org/10.1186/s12890-022-01919-x |
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