MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome sequences from 160 elasmobranchs (sharks and ray...

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Bibliographic Details
Main Authors: M. Miya, Y. Sato, T. Fukunaga, T. Sado, J. Y. Poulsen, K. Sato, T. Minamoto, S. Yamamoto, H. Yamanaka, H. Araki, M. Kondoh, W. Iwasaki
Format: Article
Language:English
Published: The Royal Society 2015-01-01
Series:Royal Society Open Science
Subjects:
metabarcoding
miseq
environmental dna
mitogenome
resource management
community ecology
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.150088

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https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.150088

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