Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry
Mycotoxins are common food contaminants which cause poisoning and severe health risks to humans and animals. The present study applied chemometric approach in liquid chromatography-tandem mass spectrometry (LC-MS/MS) optimization for simultaneous determination of mycotoxins, i.e., aflatoxins B1, B2,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/4/196 |
| _version_ | 1797999439690858496 |
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| author | Ali Mohamed Ali Alsharif Yeun-Mun Choo Guan-Huat Tan |
| author_facet | Ali Mohamed Ali Alsharif Yeun-Mun Choo Guan-Huat Tan |
| author_sort | Ali Mohamed Ali Alsharif |
| collection | DOAJ |
| description | Mycotoxins are common food contaminants which cause poisoning and severe health risks to humans and animals. The present study applied chemometric approach in liquid chromatography-tandem mass spectrometry (LC-MS/MS) optimization for simultaneous determination of mycotoxins, i.e., aflatoxins B1, B2, G1, and G2, and ochratoxin A. The validated quick, easy, cheap, effective, rugged, and safe (QuEChERS)-LC-MS/MS method was used to study the occurrence of mycotoxins in 120 food matrices. The recovery ranges from 81.94% to 101.67% with relative standard deviation (RSD) lesser than 11%. Through the developed method, aflatoxins were detected in raisin, pistachio, peanut, wheat flour, spice, and chili samples with concentration ranges from 0.45 to 16.93 µg/kg. Trace concentration of ochratoxin A was found in wheat flour and peanut samples which ranged from 1.2 to 3.53 µg/kg. Some of the tested food samples contained mycotoxins of above the European legal maximum limit. |
| first_indexed | 2024-04-11T11:04:38Z |
| format | Article |
| id | doaj.art-eeab69c89893443192c92dcc19c76cf1 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T11:04:38Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-eeab69c89893443192c92dcc19c76cf12022-12-22T04:28:26ZengMDPI AGToxins2072-66512019-03-0111419610.3390/toxins11040196toxins11040196Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass SpectrometryAli Mohamed Ali Alsharif0Yeun-Mun Choo1Guan-Huat Tan2Department of Chemistry, Faculty of Science, University of Mala, Kuala Lumpur 50603, MalaysiaDepartment of Chemistry, Faculty of Science, University of Mala, Kuala Lumpur 50603, MalaysiaDepartment of Chemistry, Faculty of Science, University of Mala, Kuala Lumpur 50603, MalaysiaMycotoxins are common food contaminants which cause poisoning and severe health risks to humans and animals. The present study applied chemometric approach in liquid chromatography-tandem mass spectrometry (LC-MS/MS) optimization for simultaneous determination of mycotoxins, i.e., aflatoxins B1, B2, G1, and G2, and ochratoxin A. The validated quick, easy, cheap, effective, rugged, and safe (QuEChERS)-LC-MS/MS method was used to study the occurrence of mycotoxins in 120 food matrices. The recovery ranges from 81.94% to 101.67% with relative standard deviation (RSD) lesser than 11%. Through the developed method, aflatoxins were detected in raisin, pistachio, peanut, wheat flour, spice, and chili samples with concentration ranges from 0.45 to 16.93 µg/kg. Trace concentration of ochratoxin A was found in wheat flour and peanut samples which ranged from 1.2 to 3.53 µg/kg. Some of the tested food samples contained mycotoxins of above the European legal maximum limit.https://www.mdpi.com/2072-6651/11/4/196mycotoxinsaflatoxinochratoxinPlackett–Burman designBox–Behnken designQuEChERSLC-MS/MS |
| spellingShingle | Ali Mohamed Ali Alsharif Yeun-Mun Choo Guan-Huat Tan Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry Toxins mycotoxins aflatoxin ochratoxin Plackett–Burman design Box–Behnken design QuEChERS LC-MS/MS |
| title | Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry |
| title_full | Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry |
| title_fullStr | Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry |
| title_full_unstemmed | Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry |
| title_short | Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry |
| title_sort | detection of five mycotoxins in different food matrices in the malaysian market by using validated liquid chromatography electrospray ionization triple quadrupole mass spectrometry |
| topic | mycotoxins aflatoxin ochratoxin Plackett–Burman design Box–Behnken design QuEChERS LC-MS/MS |
| url | https://www.mdpi.com/2072-6651/11/4/196 |
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