Adapter dimer contamination in sRNA‐sequencing datasets predicts sequencing failure and batch effects and hampers extracellular vesicle‐sRNA analysis

Adapter dimer contamination in sRNA‐sequencing datasets predicts sequencing failure and batch effects and hampers extracellular vesicle‐sRNA analysis

Abstract Small RNA (sRNA) profiling of Extracellular Vesicles (EVs) by Next‐Generation Sequencing (NGS) often delivers poor outcomes, independently of reagents, platforms or pipelines used, which contributes to poor reproducibility of studies. Here we analysed pre/post‐sequencing quality controls (Q...

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Bibliographic Details
Main Authors: Joaquín J. Maqueda, Alberta Giovanazzi, Ana Mafalda Rocha, Sara Rocha, Isabel Silva, Nadine Saraiva, Nuno Bonito, Joana Carvalho, Luis Maia, Marca H. M. Wauben, Carla Oliveira
Format: Article
Language:English
Published: Wiley 2023-06-01
Series:Journal of Extracellular Biology
Subjects:
adapter dimers
batch effects
extracellular vesicles
library preparation
quality control
small RNA
Online Access:https://doi.org/10.1002/jex2.91

Internet

https://doi.org/10.1002/jex2.91

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