Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis
Abstract Background The infrapatellar fat pad (IFP) is implicated in knee osteoarthritis (KOA). Calcitonin gene-related peptide (CGRP), a vasoactive neuropeptide expressed in joint tissues and synovial tissues (ST), was recently found to be associated with KOA progression and pain. CGRP is expressed...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-018-0864-8 |
| _version_ | 1828786357483339776 |
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| author | Jun Aikawa Kentaro Uchida Shotaro Takano Gen Inoue Dai Iwase Masayuki Miyagi Manabu Mukai Shintaro Shoji Hiroyuki Sekiguchi Masashi Takaso |
| author_facet | Jun Aikawa Kentaro Uchida Shotaro Takano Gen Inoue Dai Iwase Masayuki Miyagi Manabu Mukai Shintaro Shoji Hiroyuki Sekiguchi Masashi Takaso |
| author_sort | Jun Aikawa |
| collection | DOAJ |
| description | Abstract Background The infrapatellar fat pad (IFP) is implicated in knee osteoarthritis (KOA). Calcitonin gene-related peptide (CGRP), a vasoactive neuropeptide expressed in joint tissues and synovial tissues (ST), was recently found to be associated with KOA progression and pain. CGRP is expressed in the IFPs of human KOA patients; however, its regulation has not been elucidated. Methods IFPs and STs were harvested from 138 KOA patients during total knee replacement (TKR) and analyzed for CGRP, cycloxygenase-2 (COX-2), and microsomal prostaglandin E synthase-1 (mPGES-1) expression using real-time polymerase chain reaction (PCR). To investigate CGRP regulation by prostaglandin E2 (PGE2), adipocytes (Ad) and the stromal vascular fraction (SVF) were harvested from IFPs using collagenase. Synovial cells (SYC) were also harvested from ST and stimulated with vehicle (serum-free culture medium), PGE2, or CGRP. Results CGRP, COX-2, and mPGES-1 expression levels were significantly higher in IFPs than STs. PGE2 stimulation increased CGRP expression in Ad, the SVF, and SYC; however, CGRP expression was significantly higher in PGE2-stimulated SVF than PGE2-stimulated SYC. CGRP stimulation had no effect on COX-2 or mPGES-1 expression. Conclusions CGRP expression in the IFP of KOA patients is regulated by the COX-2/mPGES-1/PGE2 pathway. |
| first_indexed | 2024-12-12T00:09:56Z |
| format | Article |
| id | doaj.art-eedc56747da549808655c05fab3c9ab5 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-12T00:09:56Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-eedc56747da549808655c05fab3c9ab52022-12-22T00:45:00ZengBMCLipids in Health and Disease1476-511X2018-09-011711610.1186/s12944-018-0864-8Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritisJun Aikawa0Kentaro Uchida1Shotaro Takano2Gen Inoue3Dai Iwase4Masayuki Miyagi5Manabu Mukai6Shintaro Shoji7Hiroyuki Sekiguchi8Masashi Takaso9Department of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineDepartment of Orthopedic Surgery, Kitasato University School of MedicineShonan University of Medical Sciences Research InstituteDepartment of Orthopedic Surgery, Kitasato University School of MedicineAbstract Background The infrapatellar fat pad (IFP) is implicated in knee osteoarthritis (KOA). Calcitonin gene-related peptide (CGRP), a vasoactive neuropeptide expressed in joint tissues and synovial tissues (ST), was recently found to be associated with KOA progression and pain. CGRP is expressed in the IFPs of human KOA patients; however, its regulation has not been elucidated. Methods IFPs and STs were harvested from 138 KOA patients during total knee replacement (TKR) and analyzed for CGRP, cycloxygenase-2 (COX-2), and microsomal prostaglandin E synthase-1 (mPGES-1) expression using real-time polymerase chain reaction (PCR). To investigate CGRP regulation by prostaglandin E2 (PGE2), adipocytes (Ad) and the stromal vascular fraction (SVF) were harvested from IFPs using collagenase. Synovial cells (SYC) were also harvested from ST and stimulated with vehicle (serum-free culture medium), PGE2, or CGRP. Results CGRP, COX-2, and mPGES-1 expression levels were significantly higher in IFPs than STs. PGE2 stimulation increased CGRP expression in Ad, the SVF, and SYC; however, CGRP expression was significantly higher in PGE2-stimulated SVF than PGE2-stimulated SYC. CGRP stimulation had no effect on COX-2 or mPGES-1 expression. Conclusions CGRP expression in the IFP of KOA patients is regulated by the COX-2/mPGES-1/PGE2 pathway.http://link.springer.com/article/10.1186/s12944-018-0864-8Calcitonin gene-related peptide/SEOsteoarthritis, kneeAdipose tissue/PPProstaglandin E2 |
| spellingShingle | Jun Aikawa Kentaro Uchida Shotaro Takano Gen Inoue Dai Iwase Masayuki Miyagi Manabu Mukai Shintaro Shoji Hiroyuki Sekiguchi Masashi Takaso Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis Lipids in Health and Disease Calcitonin gene-related peptide/SE Osteoarthritis, knee Adipose tissue/PP Prostaglandin E2 |
| title | Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis |
| title_full | Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis |
| title_fullStr | Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis |
| title_full_unstemmed | Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis |
| title_short | Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis |
| title_sort | regulation of calcitonin gene related peptide expression through the cox 2 mpges 1 pge2 pathway in the infrapatellar fat pad in knee osteoarthritis |
| topic | Calcitonin gene-related peptide/SE Osteoarthritis, knee Adipose tissue/PP Prostaglandin E2 |
| url | http://link.springer.com/article/10.1186/s12944-018-0864-8 |
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