A method for determining the cutting efficiency of the CRISPR/Cas system in birch and poplar
Determination of Cas9 cutting efficiency to the target sites is important for genome editing. However, this determination can only be made via an in vitro method, as the purification of Cas protein and synthesis of gRNA are necessary. Here, we developed an in vivo method, called transient CRISPR/Cas...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2021-01-01
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| Series: | Forestry Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/FR-2021-0016 |