Reducing metabolic burden in the PACEmid evolver system by remastering high‐copy phagemid vectors
Abstract Orthogonal or non‐cross‐reacting transcription factors are used in synthetic biology as components of genetic circuits. Brödel et al. (2016) engineered 12 such cIλ transcription factor variants using a directed evolution ‘PACEmid’ system. The variants operate as dual activator/repressors an...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-06-01
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| Series: | Engineering Biology |
| Online Access: | https://doi.org/10.1049/enb2.12021 |