miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3
BackgroundMicroRNAs are known to regulate carcinogenesis of osteosarcoma. Although, miR-16-5p is known to exert inhibitory effects on several forms of cancers, its effects on the growth and invasion of osteosarcoma have not been studied.MethodsWe collected human osteosarcoma specimens and adjacent t...
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.01324/full |
| _version_ | 1818294789744885760 |
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| author | Zhijian Gu Zhikun Li Ruijun Xu Xiaodong Zhu Ruixi Hu Yonghua Xue Wei Xu |
| author_facet | Zhijian Gu Zhikun Li Ruijun Xu Xiaodong Zhu Ruixi Hu Yonghua Xue Wei Xu |
| author_sort | Zhijian Gu |
| collection | DOAJ |
| description | BackgroundMicroRNAs are known to regulate carcinogenesis of osteosarcoma. Although, miR-16-5p is known to exert inhibitory effects on several forms of cancers, its effects on the growth and invasion of osteosarcoma have not been studied.MethodsWe collected human osteosarcoma specimens and adjacent tissues to detect the expression of miR-16-5p by real-time polymerase chain reaction, immunoblotting, and immunohistochemistry. The proliferation, migration, and invasion of MG63 and HOS cells following miR-16-5p overexpression and inhibition were detected with cell counting kit-8, wound healing assay, and Transwell assay, respectively. An expression vector carrying a mutated 3′-untranslated region of mothers against decapentaplegic homolog 3 (Smad3) was constructed.ResultsThe results showed that miR-16-5p expression was downregulated in osteosarcoma tissues and cells as compared with adjacent counterparts, while Smad3 was overexpressed in osteosarcoma cells. The overexpression of miR-16-5p resulted in the inhibition of the proliferation, migration, and invasion of osteosarcoma cells and enhanced the therapeutic effect of cisplatin. These effects were attenuated with miR-16-5p expression inhibition. In cells transfected with miR-16-5p mimic, Smad3 expression decreased, while this effect was absent in the cells carrying mutated Smad3.ConclusionsTherefore, miR-16-5p inhibits the growth and invasion of osteosarcoma by targeting Smad3. |
| first_indexed | 2024-12-13T03:37:20Z |
| format | Article |
| id | doaj.art-ef547cb5c95144289a2abdaebd637329 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-13T03:37:20Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-ef547cb5c95144289a2abdaebd6373292022-12-22T00:01:01ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-08-011110.3389/fphar.2020.01324534070miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3Zhijian Gu0Zhikun Li1Ruijun Xu2Xiaodong Zhu3Ruixi Hu4Yonghua Xue5Wei Xu6Department of Orthopedics, Tongren Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Orthopedics, Tongren Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Orthopedics, Tongren Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Orthopedics, Tongren Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Orthopedics, Tongren Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurosurgery, Putuo District Central Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Orthopedics, Tongren Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaBackgroundMicroRNAs are known to regulate carcinogenesis of osteosarcoma. Although, miR-16-5p is known to exert inhibitory effects on several forms of cancers, its effects on the growth and invasion of osteosarcoma have not been studied.MethodsWe collected human osteosarcoma specimens and adjacent tissues to detect the expression of miR-16-5p by real-time polymerase chain reaction, immunoblotting, and immunohistochemistry. The proliferation, migration, and invasion of MG63 and HOS cells following miR-16-5p overexpression and inhibition were detected with cell counting kit-8, wound healing assay, and Transwell assay, respectively. An expression vector carrying a mutated 3′-untranslated region of mothers against decapentaplegic homolog 3 (Smad3) was constructed.ResultsThe results showed that miR-16-5p expression was downregulated in osteosarcoma tissues and cells as compared with adjacent counterparts, while Smad3 was overexpressed in osteosarcoma cells. The overexpression of miR-16-5p resulted in the inhibition of the proliferation, migration, and invasion of osteosarcoma cells and enhanced the therapeutic effect of cisplatin. These effects were attenuated with miR-16-5p expression inhibition. In cells transfected with miR-16-5p mimic, Smad3 expression decreased, while this effect was absent in the cells carrying mutated Smad3.ConclusionsTherefore, miR-16-5p inhibits the growth and invasion of osteosarcoma by targeting Smad3.https://www.frontiersin.org/article/10.3389/fphar.2020.01324/fullosteosarcomamiR-16-5pinvasionSmad3proliferation |
| spellingShingle | Zhijian Gu Zhikun Li Ruijun Xu Xiaodong Zhu Ruixi Hu Yonghua Xue Wei Xu miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3 Frontiers in Pharmacology osteosarcoma miR-16-5p invasion Smad3 proliferation |
| title | miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3 |
| title_full | miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3 |
| title_fullStr | miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3 |
| title_full_unstemmed | miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3 |
| title_short | miR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3 |
| title_sort | mir 16 5p suppresses progression and invasion of osteosarcoma via targeting at smad3 |
| topic | osteosarcoma miR-16-5p invasion Smad3 proliferation |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.01324/full |
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