CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in <named-content content-type="genus-species">Trypanosoma cruzi</named-content> Reveals Their Role in Flagellar Attachment
ABSTRACT Trypanosoma cruzi is the etiologic agent of Chagas disease, and current methods for its genetic manipulation have been highly inefficient. We report here the use of the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated gene 9) system for disrupting g...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2015-09-01
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| Series: | mBio |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.01012-15 |