Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach
This study investigates the potential of utilizing three food wastes: cheese whey (CW), beet molasses (BM), and corn steep liquor (CSL) as alternative nutrient sources for the cultivation of the diatom <i>Phaeodactylum tricornutum</i>, a promising source of polyunsaturated eicosapentaeno...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/3/190 |
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| author | Giovanni Luca Russo Antonio Luca Langellotti Vito Verardo Beatriz Martín-García Maria Oliviero Marco Baselice Prospero Di Pierro Angela Sorrentino Sharon Viscardi Luis Marileo Raffaele Sacchi Paolo Masi |
| author_facet | Giovanni Luca Russo Antonio Luca Langellotti Vito Verardo Beatriz Martín-García Maria Oliviero Marco Baselice Prospero Di Pierro Angela Sorrentino Sharon Viscardi Luis Marileo Raffaele Sacchi Paolo Masi |
| author_sort | Giovanni Luca Russo |
| collection | DOAJ |
| description | This study investigates the potential of utilizing three food wastes: cheese whey (CW), beet molasses (BM), and corn steep liquor (CSL) as alternative nutrient sources for the cultivation of the diatom <i>Phaeodactylum tricornutum</i>, a promising source of polyunsaturated eicosapentaenoic acid (EPA) and the carotenoid fucoxanthin. The CW media tested did not significantly impact the growth rate of <i>P. tricornutum</i>; however, CW hydrolysate significantly enhances cell growth. BM in cultivation medium enhances biomass production and fucoxanthin yield. The optimization of the new food waste medium was conducted through the application of a response surface methodology (RSM) using hydrolyzed CW, BM, and CSL as factors. The results showed a significant positive impact of these factors (<i>p</i> < 0.005), with an optimized biomass yield of 2.35 g L<sup>−1</sup> and a fucoxanthin yield of 3.64 mg L<sup>−1</sup> using a medium composed of 33 mL L<sup>−1</sup> of CW, 2.3 g L<sup>−1</sup> of BM, and 2.24 g L<sup>−1</sup> of CSL. The experimental results reported in this study showed that some food by-products from a biorefinery perspective could be utilized for the efficient production of fucoxanthin and other high-added-value products such as eicosapentaenoic acid (EPA). |
| first_indexed | 2024-03-11T06:16:47Z |
| format | Article |
| id | doaj.art-ef8ac464bbab485b82702b59b6106ad1 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T06:16:47Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-ef8ac464bbab485b82702b59b6106ad12023-11-17T12:17:49ZengMDPI AGMarine Drugs1660-33972023-03-0121319010.3390/md21030190Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery ApproachGiovanni Luca Russo0Antonio Luca Langellotti1Vito Verardo2Beatriz Martín-García3Maria Oliviero4Marco Baselice5Prospero Di Pierro6Angela Sorrentino7Sharon Viscardi8Luis Marileo9Raffaele Sacchi10Paolo Masi11CAISIAL Center, University of Naples Federico II, Via Università 133, 80055 Portici, ItalyCAISIAL Center, University of Naples Federico II, Via Università 133, 80055 Portici, ItalyDepartment of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 Granada, SpainDepartment of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 Granada, SpainDepartment of Animal Health, Experimental Zooprophylactic Institute of Southern Italy, Via Salute, 2, 80055 Portici, ItalyDepartment of Civil, Environmental, Land, Construction and Chemistry (DICATECh), Politecnico di Bari, 70126 Bari, ItalyDepartment of Agricultural Sciences, Unit of Food Science and Technology, University of Naples Federico II, 80055 Portici, ItalyCAISIAL Center, University of Naples Federico II, Via Università 133, 80055 Portici, ItalyBiotechnology of Functional Foods Laboratory, Camino Sanquilco, Parcela 18, Padre Las Casas 4850827, La Araucanía, ChileBiotechnology of Functional Foods Laboratory, Camino Sanquilco, Parcela 18, Padre Las Casas 4850827, La Araucanía, ChileDepartment of Agricultural Sciences, Unit of Food Science and Technology, University of Naples Federico II, 80055 Portici, ItalyCAISIAL Center, University of Naples Federico II, Via Università 133, 80055 Portici, ItalyThis study investigates the potential of utilizing three food wastes: cheese whey (CW), beet molasses (BM), and corn steep liquor (CSL) as alternative nutrient sources for the cultivation of the diatom <i>Phaeodactylum tricornutum</i>, a promising source of polyunsaturated eicosapentaenoic acid (EPA) and the carotenoid fucoxanthin. The CW media tested did not significantly impact the growth rate of <i>P. tricornutum</i>; however, CW hydrolysate significantly enhances cell growth. BM in cultivation medium enhances biomass production and fucoxanthin yield. The optimization of the new food waste medium was conducted through the application of a response surface methodology (RSM) using hydrolyzed CW, BM, and CSL as factors. The results showed a significant positive impact of these factors (<i>p</i> < 0.005), with an optimized biomass yield of 2.35 g L<sup>−1</sup> and a fucoxanthin yield of 3.64 mg L<sup>−1</sup> using a medium composed of 33 mL L<sup>−1</sup> of CW, 2.3 g L<sup>−1</sup> of BM, and 2.24 g L<sup>−1</sup> of CSL. The experimental results reported in this study showed that some food by-products from a biorefinery perspective could be utilized for the efficient production of fucoxanthin and other high-added-value products such as eicosapentaenoic acid (EPA).https://www.mdpi.com/1660-3397/21/3/190PUFAsustainabilityfood by-productbioconversionbiorefinerydairy wastewater |
| spellingShingle | Giovanni Luca Russo Antonio Luca Langellotti Vito Verardo Beatriz Martín-García Maria Oliviero Marco Baselice Prospero Di Pierro Angela Sorrentino Sharon Viscardi Luis Marileo Raffaele Sacchi Paolo Masi Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach Marine Drugs PUFA sustainability food by-product bioconversion biorefinery dairy wastewater |
| title | Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach |
| title_full | Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach |
| title_fullStr | Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach |
| title_full_unstemmed | Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach |
| title_short | Bioconversion of Cheese Whey and Food By-Products by <i>Phaeodactylum tricornutum</i> into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach |
| title_sort | bioconversion of cheese whey and food by products by i phaeodactylum tricornutum i into fucoxanthin and n 3 lc pufa through a biorefinery approach |
| topic | PUFA sustainability food by-product bioconversion biorefinery dairy wastewater |
| url | https://www.mdpi.com/1660-3397/21/3/190 |
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